Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves
Soybean, a vital protein-rich crop, offers bioactivity that can mitigate various chronic human diseases. Nonetheless, soybean breeding poses a challenge due to the negative correlation between enhanced protein levels and overall productivity. Our previous studies demonstrated that applying gaseous p...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2023.2287883 |
| _version_ | 1797351902808113152 |
|---|---|
| author | Gyeongik Ahn Yeong Jun Ban Gyeong-Im Shin Song Yi Jeong Ki Hun Park Woe-Yeon Kim Joon-Yung Cha |
| author_facet | Gyeongik Ahn Yeong Jun Ban Gyeong-Im Shin Song Yi Jeong Ki Hun Park Woe-Yeon Kim Joon-Yung Cha |
| author_sort | Gyeongik Ahn |
| collection | DOAJ |
| description | Soybean, a vital protein-rich crop, offers bioactivity that can mitigate various chronic human diseases. Nonetheless, soybean breeding poses a challenge due to the negative correlation between enhanced protein levels and overall productivity. Our previous studies demonstrated that applying gaseous phytohormone, ethylene, to soybean leaves significantly boosts the accumulation of free amino acids, particularly asparagine (Asn). Current studies also revealed that ethylene application to soybeans significantly enhanced both essential and non-essential amino acid contents in leaves and stems. Asn plays a crucial role in ammonia detoxification and reducing fatigue. However, the molecular evidence supporting this phenomenon remains elusive. This study explores the molecular mechanisms behind enhanced Asn accumulation in ethylene-treated soybean leaves. Transcriptional analysis revealed that ethylene treatments to soybean leaves enhance the transcriptional levels of key genes involved in Asn biosynthesis, such as aspartate aminotransferase (AspAT) and Asn synthetase (ASN), which aligns with our previous observations of elevated Asn levels. These findings shed light on the role of ethylene in upregulating Asn biosynthetic genes, subsequently enhancing Asn concentrations. This molecular insight into amino acid metabolism regulation provides valuable knowledge for the metabolic farming of crops, especially in elevating nutraceutical ingredients with non-genetic modification (GM) approach for improved protein content. |
| first_indexed | 2024-03-08T13:08:27Z |
| format | Article |
| id | doaj.art-8abb76fae02d462eb2bc2f776b4719fa |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:27Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-8abb76fae02d462eb2bc2f776b4719fa2024-01-18T15:58:23ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242023-12-0118110.1080/15592324.2023.22878832287883Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leavesGyeongik Ahn0Yeong Jun Ban1Gyeong-Im Shin2Song Yi Jeong3Ki Hun Park4Woe-Yeon Kim5Joon-Yung Cha6Gyeongsang National UniversityKorea Institute of Oriental MedicineGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversitySoybean, a vital protein-rich crop, offers bioactivity that can mitigate various chronic human diseases. Nonetheless, soybean breeding poses a challenge due to the negative correlation between enhanced protein levels and overall productivity. Our previous studies demonstrated that applying gaseous phytohormone, ethylene, to soybean leaves significantly boosts the accumulation of free amino acids, particularly asparagine (Asn). Current studies also revealed that ethylene application to soybeans significantly enhanced both essential and non-essential amino acid contents in leaves and stems. Asn plays a crucial role in ammonia detoxification and reducing fatigue. However, the molecular evidence supporting this phenomenon remains elusive. This study explores the molecular mechanisms behind enhanced Asn accumulation in ethylene-treated soybean leaves. Transcriptional analysis revealed that ethylene treatments to soybean leaves enhance the transcriptional levels of key genes involved in Asn biosynthesis, such as aspartate aminotransferase (AspAT) and Asn synthetase (ASN), which aligns with our previous observations of elevated Asn levels. These findings shed light on the role of ethylene in upregulating Asn biosynthetic genes, subsequently enhancing Asn concentrations. This molecular insight into amino acid metabolism regulation provides valuable knowledge for the metabolic farming of crops, especially in elevating nutraceutical ingredients with non-genetic modification (GM) approach for improved protein content.http://dx.doi.org/10.1080/15592324.2023.2287883asn synthetaseasparagine biosynthesisaspartate aminotransferaseethylenesoybean |
| spellingShingle | Gyeongik Ahn Yeong Jun Ban Gyeong-Im Shin Song Yi Jeong Ki Hun Park Woe-Yeon Kim Joon-Yung Cha Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves Plant Signaling & Behavior asn synthetase asparagine biosynthesis aspartate aminotransferase ethylene soybean |
| title | Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves |
| title_full | Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves |
| title_fullStr | Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves |
| title_full_unstemmed | Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves |
| title_short | Ethylene enhances transcriptions of asparagine biosynthetic genes in soybean (Glycine max L. Merr) leaves |
| title_sort | ethylene enhances transcriptions of asparagine biosynthetic genes in soybean glycine max l merr leaves |
| topic | asn synthetase asparagine biosynthesis aspartate aminotransferase ethylene soybean |
| url | http://dx.doi.org/10.1080/15592324.2023.2287883 |
| work_keys_str_mv | AT gyeongikahn ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT yeongjunban ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT gyeongimshin ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT songyijeong ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT kihunpark ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT woeyeonkim ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves AT joonyungcha ethyleneenhancestranscriptionsofasparaginebiosyntheticgenesinsoybeanglycinemaxlmerrleaves |