Bacterial diversity in the sediments of a temperate artificial lake, Rapel reservoir Diversidad bacteriana en sedimentos de un lago artificial temperado, embalse Rapel
Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-06-01
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| Series: | Revista Chilena de Historia Natural |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000200007 |
| Summary: | Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques and molecular biology tools. Our results showed significant temporal variation in the physical and chemical composition of the water column, but no depth-related differences during the study period. To detect temporal changes in bacterial composition, cultivable heterotrophic bacteria, heterotrophic iron oxidizing bacteria, and sulfate-reducing bacteria were extracted from the surface sediments and their concentration measured. Microbial diversity in sediments was represented by closest relatives of eight different bacterial phyla. The most frequently recovered phylotypes in the clone library of 16S rDNA were related to sulfate-reducing bacteria belong to the Deltaproteobacteria group<br>El embalse Rapel es un sistema eutrófico en Chile que ha experimentado diversos impactos antropogénicos en las últimas décadas. Para conocer mejor los posibles procesos que ocurren en el embalse, la composición bacteriana fue estudiada en la zona superficial de los sedimentos utilizando técnicas microbiológicas tradicionales y herramientas de biología molecular. Nuestros resultados mostraron variación temporal significativa en la composición física y química del agua, no encontrándose diferencias de las variables medidas entre superficie y fondo en la columna de agua durante el período de estudio. Para detectar cambios temporales en la concentración y composición bacteriana, se midieron bacterias heterótrofas, heterótrofas oxidantes de fierro y bacterias reductoras de sulfato extraídas de la superficie de los sedimentos. La diversidad bacteriana en los sedimentos estuvo representada por 8 diferentes Phyla del dominio Bacteria. Los filotipos más frecuentes en la genoteca estuvieron relacionados a bacterias reductoras de sulfato pertenecientes al grupo de Deltaproteobacteria |
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| ISSN: | 0716-078X 0717-6317 |