Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study
Hericium erinaceus, berberine, and quercetin are effective in experimental colitis. It is unknown whether they can ameliorate inflammatory bowel diseases in humans. This ex vivo study aimed to evaluate the anti-inflammatory potential of a nutraceutical compound of HBQ-Complex® (H. erinaceus, berberi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1215329/full |
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| author | Antonietta Gerarda Gravina Raffaele Pellegrino Giovanna Palladino Annachiara Coppola Giovanni Brandimarte Concetta Tuccillo Fortunato Ciardiello Marco Romano Alessandro Federico |
| author_facet | Antonietta Gerarda Gravina Raffaele Pellegrino Giovanna Palladino Annachiara Coppola Giovanni Brandimarte Concetta Tuccillo Fortunato Ciardiello Marco Romano Alessandro Federico |
| author_sort | Antonietta Gerarda Gravina |
| collection | DOAJ |
| description | Hericium erinaceus, berberine, and quercetin are effective in experimental colitis. It is unknown whether they can ameliorate inflammatory bowel diseases in humans. This ex vivo study aimed to evaluate the anti-inflammatory potential of a nutraceutical compound of HBQ-Complex® (H. erinaceus, berberine, and quercetin), biotin, and niacin in inflammatory bowel disease patients. Tissue specimens were obtained either from Normal-Appearing Mucosa (NAM) or from Inflamed Mucosa (IM) in 20 patients with inflammatory bowel disease. mRNA and protein expression of COX-2, IL-10, and TNF-α were determined in NAM and IM biopsy samples (T0). IM samples were then incubated in HBQ-Complex® (with the addition of niacin and biotin), and COX-2, IL-10, and TNF-α tissue levels were evaluated at 120 minutes (T1) and 180 minutes (T2). Incubation with this compound resulted in a progressive decrease in gene and protein COX-2 and TNF-α expression at T1/T2 in the IM. IL-10 showed an opposite trend, with a progressive increase of mRNA and protein expression over the same time window. HBQ-Complex® (with the addition of niacin and biotin) decreased the expression of proinflammatory cytokines at the mRNA and protein levels in IBD tissue. On the contrary, mRNA and protein expression of the anti-inflammatory cytokine IL-10 showed a progressive increase. |
| first_indexed | 2024-03-13T01:47:42Z |
| format | Article |
| id | doaj.art-8abde5910515439d9857ba0a3bd901a6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T01:47:42Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8abde5910515439d9857ba0a3bd901a62023-07-03T05:47:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-07-011410.3389/fimmu.2023.12153291215329Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo studyAntonietta Gerarda Gravina0Raffaele Pellegrino1Giovanna Palladino2Annachiara Coppola3Giovanni Brandimarte4Concetta Tuccillo5Fortunato Ciardiello6Marco Romano7Alessandro Federico8Hepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyDivision of Internal Medicine and Gastroenterology, Cristo Re Hospital, Rome, ItalyMedical Oncology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyMedical Oncology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHepatogastroenterology Unit, Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, ItalyHericium erinaceus, berberine, and quercetin are effective in experimental colitis. It is unknown whether they can ameliorate inflammatory bowel diseases in humans. This ex vivo study aimed to evaluate the anti-inflammatory potential of a nutraceutical compound of HBQ-Complex® (H. erinaceus, berberine, and quercetin), biotin, and niacin in inflammatory bowel disease patients. Tissue specimens were obtained either from Normal-Appearing Mucosa (NAM) or from Inflamed Mucosa (IM) in 20 patients with inflammatory bowel disease. mRNA and protein expression of COX-2, IL-10, and TNF-α were determined in NAM and IM biopsy samples (T0). IM samples were then incubated in HBQ-Complex® (with the addition of niacin and biotin), and COX-2, IL-10, and TNF-α tissue levels were evaluated at 120 minutes (T1) and 180 minutes (T2). Incubation with this compound resulted in a progressive decrease in gene and protein COX-2 and TNF-α expression at T1/T2 in the IM. IL-10 showed an opposite trend, with a progressive increase of mRNA and protein expression over the same time window. HBQ-Complex® (with the addition of niacin and biotin) decreased the expression of proinflammatory cytokines at the mRNA and protein levels in IBD tissue. On the contrary, mRNA and protein expression of the anti-inflammatory cytokine IL-10 showed a progressive increase.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1215329/fullinflammatory bowel diseaseHericium erinaceusquercetinberberinbiotinniacin |
| spellingShingle | Antonietta Gerarda Gravina Raffaele Pellegrino Giovanna Palladino Annachiara Coppola Giovanni Brandimarte Concetta Tuccillo Fortunato Ciardiello Marco Romano Alessandro Federico Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study Frontiers in Immunology inflammatory bowel disease Hericium erinaceus quercetin berberin biotin niacin |
| title | Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study |
| title_full | Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study |
| title_fullStr | Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study |
| title_full_unstemmed | Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study |
| title_short | Hericium erinaceus, in combination with natural flavonoid/alkaloid and B3/B8 vitamins, can improve inflammatory burden in Inflammatory bowel diseases tissue: an ex vivo study |
| title_sort | hericium erinaceus in combination with natural flavonoid alkaloid and b3 b8 vitamins can improve inflammatory burden in inflammatory bowel diseases tissue an ex vivo study |
| topic | inflammatory bowel disease Hericium erinaceus quercetin berberin biotin niacin |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1215329/full |
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