Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L.
The use of in vitro techniques for the production of secondary metabolites in the pharmaceutical industry has increased widely. The organ culture is one of the alternatives for the identification of new compounds or to increase the production of already known compound useful for the humanity. The ai...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2009-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/306 |
| _version_ | 1819028286171250688 |
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| author | Alina Capote Naivy Pérez-Alonso Anabel Pérez Dirk Wilken André Gerth Lutz Müller-Kuhrt Ortelio Jiménez |
| author_facet | Alina Capote Naivy Pérez-Alonso Anabel Pérez Dirk Wilken André Gerth Lutz Müller-Kuhrt Ortelio Jiménez |
| author_sort | Alina Capote |
| collection | DOAJ |
| description | The use of in vitro techniques for the production of secondary metabolites in the pharmaceutical industry has increased widely. The organ culture is one of the alternatives for the identification of new compounds or to increase the production of already known compound useful for the humanity. The aim of this work was to produce biomass of Psidium guajava L var ‘EEA18-40’ using shoot culture and to determine the effect of the in vitro culture in the metabolic profile of this spicies. Water content was higher in the plant material obtained in the shoots cultured in vitro compared to samples collected from plants grown in field. Yields of extracts were greater in shoots cultured in vitro. Expression profiles among leaves samples of plants grown in field were different in this species, obtaining a higher rate in the shoots culture.A number of 23 new compounds was detected . This is the first work that shows the metabolic profile of Psidium guajava in shoots cultured in vitro. It was demonstrated that the in vitro culture is a potential source for identification and production of new compounds.
Key words: guava, mass spectrometry, organs culture, secondary metabolites |
| first_indexed | 2024-12-21T05:55:57Z |
| format | Article |
| id | doaj.art-8ac2ce1835764058a02ce0860dbb6634 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-21T05:55:57Z |
| publishDate | 2009-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-8ac2ce1835764058a02ce0860dbb66342022-12-21T19:13:52ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472009-01-0191280Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L.Alina CapoteNaivy Pérez-AlonsoAnabel PérezDirk WilkenAndré GerthLutz Müller-KuhrtOrtelio JiménezThe use of in vitro techniques for the production of secondary metabolites in the pharmaceutical industry has increased widely. The organ culture is one of the alternatives for the identification of new compounds or to increase the production of already known compound useful for the humanity. The aim of this work was to produce biomass of Psidium guajava L var ‘EEA18-40’ using shoot culture and to determine the effect of the in vitro culture in the metabolic profile of this spicies. Water content was higher in the plant material obtained in the shoots cultured in vitro compared to samples collected from plants grown in field. Yields of extracts were greater in shoots cultured in vitro. Expression profiles among leaves samples of plants grown in field were different in this species, obtaining a higher rate in the shoots culture.A number of 23 new compounds was detected . This is the first work that shows the metabolic profile of Psidium guajava in shoots cultured in vitro. It was demonstrated that the in vitro culture is a potential source for identification and production of new compounds. Key words: guava, mass spectrometry, organs culture, secondary metaboliteshttps://revista.ibp.co.cu/index.php/BV/article/view/306 |
| spellingShingle | Alina Capote Naivy Pérez-Alonso Anabel Pérez Dirk Wilken André Gerth Lutz Müller-Kuhrt Ortelio Jiménez Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. Biotecnología Vegetal |
| title | Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. |
| title_full | Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. |
| title_fullStr | Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. |
| title_full_unstemmed | Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. |
| title_short | Effect of <em>in vitro</em> culture on metabolic profiling of <em>Psidium guajava</em> L. |
| title_sort | effect of em in vitro em culture on metabolic profiling of em psidium guajava em l |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/306 |
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