Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo
Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1997-03-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014 |
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| author | A.H. Straus V.B. Valero C.M. Takizawa S.B. Levery M.S. Toledo E. Suzuki M.E.K. Salyan S. Hakomori C.L. Barbieri H.K. Takahashi |
| author_facet | A.H. Straus V.B. Valero C.M. Takizawa S.B. Levery M.S. Toledo E. Suzuki M.E.K. Salyan S. Hakomori C.L. Barbieri H.K. Takahashi |
| author_sort | A.H. Straus |
| collection | DOAJ |
| description | Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to glycosphingolipids with longer carbohydrate chains, with five or more sugar units (slow migrating bands on HPTLC). Macrophages isolated from footpad lesions of BALB/c mice infected with Leishmania (L.) amazonensis were incubated with MoAb ST-3 and, by indirect immunofluorescence, labeling was only detected on the parasite, whereas no fluorescence was observed on the surface of the infected macrophages, indicating that these glycosphingolipid antigens are not acquired from the host cell but synthesized by the amastigote. Intravenous administration of 125I-labeled ST-3 antibody to infected BALB/c mice showed that MoAb ST-3 accumulated significantly in the footpad lesions in comparison to blood and other tissues |
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| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T16:51:18Z |
| publishDate | 1997-03-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-8ac332220f164b4ca12970ed64a6df6c2022-12-22T02:38:56ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1997-03-0130339510.1590/S0100-879X1997000300014Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivoA.H. StrausV.B. ValeroC.M. TakizawaS.B. LeveryM.S. ToledoE. SuzukiM.E.K. SalyanS. HakomoriC.L. BarbieriH.K. TakahashiSpecific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to glycosphingolipids with longer carbohydrate chains, with five or more sugar units (slow migrating bands on HPTLC). Macrophages isolated from footpad lesions of BALB/c mice infected with Leishmania (L.) amazonensis were incubated with MoAb ST-3 and, by indirect immunofluorescence, labeling was only detected on the parasite, whereas no fluorescence was observed on the surface of the infected macrophages, indicating that these glycosphingolipid antigens are not acquired from the host cell but synthesized by the amastigote. Intravenous administration of 125I-labeled ST-3 antibody to infected BALB/c mice showed that MoAb ST-3 accumulated significantly in the footpad lesions in comparison to blood and other tissueshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014glycosphingolipidsmonoclonal antibodiesLeishmania (L.) amazonensisfast atom bombardment mass spectrometrycarbohydrate antigens |
| spellingShingle | A.H. Straus V.B. Valero C.M. Takizawa S.B. Levery M.S. Toledo E. Suzuki M.E.K. Salyan S. Hakomori C.L. Barbieri H.K. Takahashi Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo Brazilian Journal of Medical and Biological Research glycosphingolipids monoclonal antibodies Leishmania (L.) amazonensis fast atom bombardment mass spectrometry carbohydrate antigens |
| title | Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo |
| title_full | Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo |
| title_fullStr | Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo |
| title_full_unstemmed | Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo |
| title_short | Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo |
| title_sort | glycosphingolipid antigens from leishmania l amazonensis amastigotes binding of anti glycosphingolipid monoclonal antibodies in vitro and in vivo |
| topic | glycosphingolipids monoclonal antibodies Leishmania (L.) amazonensis fast atom bombardment mass spectrometry carbohydrate antigens |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014 |
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