The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications
Anti-glaucoma eye drop treatment often induces dry eyes and can lead to poor medication adherence. This study aimed to investigate the effects of 3% diquafosol sodium eye drops on tear function and the ocular surface epithelium in <i>Sod1</i><sup>−/−</sup>...
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MDPI AG
2020-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/1/20 |
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| author | Yukari Yagi-Yaguchi Takashi Kojima Kazunari Higa Murat Dogru Osama MA. Ibrahim Takahiko Shimizu Kazuo Tsubota Jun Shimazaki |
| author_facet | Yukari Yagi-Yaguchi Takashi Kojima Kazunari Higa Murat Dogru Osama MA. Ibrahim Takahiko Shimizu Kazuo Tsubota Jun Shimazaki |
| author_sort | Yukari Yagi-Yaguchi |
| collection | DOAJ |
| description | Anti-glaucoma eye drop treatment often induces dry eyes and can lead to poor medication adherence. This study aimed to investigate the effects of 3% diquafosol sodium eye drops on tear function and the ocular surface epithelium in <i>Sod1</i><sup>−/−</sup> mice after treatment with anti-glaucoma eye drops. The mice were divided into four groups: group 1, control group; group 2, anti-glaucoma eye drop; group 3, anti-glaucoma eye drops followed by a secretagogue eye drop (3% diquafosol); and group 4, simultaneous anti-glaucoma and secretagogue eye drop. Mice underwent assessments of tear quantity, tear film breakup time, and vital staining score. Mice in groups 3 and 4 showed significantly better tear stability and lower corneal staining scores than mice in group 2 after eye drop instillations (<i>p</i> < 0.05). Mice in group 4 showed significantly better tear stability, lower corneal staining scores, and higher goblet cell densities than those in group 1 after eye drop instillations (p < 0.05). The conjunctival epithelium showed stratification and abundance of Muc5AC-positive goblet cells in group 4, whereas thinning with desquamation was observed with a few goblet cells in group 2. Thus, simultaneous administration of 3% diquafosol sodium eye drops with topical anti-glaucoma drops showed favorable effects on tear stability and the corneal epithelium against the ocular surface toxicity inflicted by the anti-glaucoma eye drops. |
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| language | English |
| last_indexed | 2024-04-14T00:47:16Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-8ac3d1b0a10143e6887957f6cf7db0fb2022-12-22T02:21:58ZengMDPI AGDiagnostics2075-44182020-01-011012010.3390/diagnostics10010020diagnostics10010020The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye MedicationsYukari Yagi-Yaguchi0Takashi Kojima1Kazunari Higa2Murat Dogru3Osama MA. Ibrahim4Takahiko Shimizu5Kazuo Tsubota6Jun Shimazaki7Department of Ophthalmology, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, JapanDepartment of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Ophthalmology, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, JapanDepartment of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Ophthalmology, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, JapanDepartment of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, JapanDepartment of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Ophthalmology, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, JapanAnti-glaucoma eye drop treatment often induces dry eyes and can lead to poor medication adherence. This study aimed to investigate the effects of 3% diquafosol sodium eye drops on tear function and the ocular surface epithelium in <i>Sod1</i><sup>−/−</sup> mice after treatment with anti-glaucoma eye drops. The mice were divided into four groups: group 1, control group; group 2, anti-glaucoma eye drop; group 3, anti-glaucoma eye drops followed by a secretagogue eye drop (3% diquafosol); and group 4, simultaneous anti-glaucoma and secretagogue eye drop. Mice underwent assessments of tear quantity, tear film breakup time, and vital staining score. Mice in groups 3 and 4 showed significantly better tear stability and lower corneal staining scores than mice in group 2 after eye drop instillations (<i>p</i> < 0.05). Mice in group 4 showed significantly better tear stability, lower corneal staining scores, and higher goblet cell densities than those in group 1 after eye drop instillations (p < 0.05). The conjunctival epithelium showed stratification and abundance of Muc5AC-positive goblet cells in group 4, whereas thinning with desquamation was observed with a few goblet cells in group 2. Thus, simultaneous administration of 3% diquafosol sodium eye drops with topical anti-glaucoma drops showed favorable effects on tear stability and the corneal epithelium against the ocular surface toxicity inflicted by the anti-glaucoma eye drops.https://www.mdpi.com/2075-4418/10/1/20dry eyeanti-glaucoma eye drops3% diquafosol sodium eye drops<i>sod1</i><sup>−/−</sup> mice |
| spellingShingle | Yukari Yagi-Yaguchi Takashi Kojima Kazunari Higa Murat Dogru Osama MA. Ibrahim Takahiko Shimizu Kazuo Tsubota Jun Shimazaki The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications Diagnostics dry eye anti-glaucoma eye drops 3% diquafosol sodium eye drops <i>sod1</i><sup>−/−</sup> mice |
| title | The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications |
| title_full | The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications |
| title_fullStr | The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications |
| title_full_unstemmed | The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications |
| title_short | The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Medications |
| title_sort | effects of 3 diquafosol sodium eye drops on tear function and the ocular surface of cu zn superoxide dismutase 1 i sod1 i knockout mice treated with antiglaucoma eye medications |
| topic | dry eye anti-glaucoma eye drops 3% diquafosol sodium eye drops <i>sod1</i><sup>−/−</sup> mice |
| url | https://www.mdpi.com/2075-4418/10/1/20 |
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