Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.)
The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612990 |
| _version_ | 1819070189831979008 |
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| author | Elsheikh Y M Ahmed Yan-pei ZHANG Jian-ping YU Rashid M A Rehman Zhan-ying ZHANG Hong-liang ZHANG Jin-jie LI Zi-chao LI |
| author_facet | Elsheikh Y M Ahmed Yan-pei ZHANG Jian-ping YU Rashid M A Rehman Zhan-ying ZHANG Hong-liang ZHANG Jin-jie LI Zi-chao LI |
| author_sort | Elsheikh Y M Ahmed |
| collection | DOAJ |
| description | The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 (indica) and Nipponbare (japonica) and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3 741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility (seed setting) and understanding the genetic bases of pollen sterility. |
| first_indexed | 2024-12-21T17:01:59Z |
| format | Article |
| id | doaj.art-8ac5aa974c1f409db8a925fab8bb9843 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T17:01:59Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-8ac5aa974c1f409db8a925fab8bb98432022-12-21T18:56:38ZengElsevierJournal of Integrative Agriculture2095-31192016-04-01154735743Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.)Elsheikh Y M Ahmed0Yan-pei ZHANG1Jian-ping YU2Rashid M A Rehman3Zhan-ying ZHANG4Hong-liang ZHANG5Jin-jie LI6Zi-chao LI7Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaKey Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaCorrespondence LI Zi-chao, Tel: +86-10-62731414; Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R. ChinaThe lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility (seed setting) is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 (indica) and Nipponbare (japonica) and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3 741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility (seed setting) and understanding the genetic bases of pollen sterility.http://www.sciencedirect.com/science/article/pii/S2095311915612990rice (Oryza sativa L.)QTL mappingseed settingpollen sterility |
| spellingShingle | Elsheikh Y M Ahmed Yan-pei ZHANG Jian-ping YU Rashid M A Rehman Zhan-ying ZHANG Hong-liang ZHANG Jin-jie LI Zi-chao LI Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) Journal of Integrative Agriculture rice (Oryza sativa L.) QTL mapping seed setting pollen sterility |
| title | Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) |
| title_full | Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) |
| title_fullStr | Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) |
| title_full_unstemmed | Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) |
| title_short | Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice (Oryza sativa L.) |
| title_sort | mapping of three qtls for seed setting and analysis on the candidate gene for qss 1 in rice oryza sativa l |
| topic | rice (Oryza sativa L.) QTL mapping seed setting pollen sterility |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612990 |
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