Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan
Abstract Background Low-dose exposure to organophosphate (OP) insecticides during pregnancy may adversely affect neurodevelopment in children. To evaluate the OP exposure levels, single urine sampling is commonly adopted to measure the levels of dialkylphosphates (DAPs), common OP metabolites. Howev...
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| Format: | Article |
| Language: | English |
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Komiyama Printing Co. Ltd
2019-01-01
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| Series: | Environmental Health and Preventive Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12199-019-0761-4 |
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| author | Keisuke Hioki Yuki Ito Naoko Oya Shoji F. Nakayama Tomohiko Isobe Takeshi Ebara Kanemitsu Shibata Naomi Nishikawa Kunihiko Nakai Tomota Kamida Jun Ueyama Mayumi Sugiura-Ogasawara Michihiro Kamijima |
| author_facet | Keisuke Hioki Yuki Ito Naoko Oya Shoji F. Nakayama Tomohiko Isobe Takeshi Ebara Kanemitsu Shibata Naomi Nishikawa Kunihiko Nakai Tomota Kamida Jun Ueyama Mayumi Sugiura-Ogasawara Michihiro Kamijima |
| author_sort | Keisuke Hioki |
| collection | DOAJ |
| description | Abstract Background Low-dose exposure to organophosphate (OP) insecticides during pregnancy may adversely affect neurodevelopment in children. To evaluate the OP exposure levels, single urine sampling is commonly adopted to measure the levels of dialkylphosphates (DAPs), common OP metabolites. However, the inter-day variations of urinary DAP concentrations within subjects are supposed to be large due to the short biological half-lives of the metabolites, and it is thus considered difficult to accurately assess OP exposure during pregnancy with single sampling. This study aimed to assess intra-individual variations of DAP concentrations and the reproducibility of the exposure dose categorization of OPs according to DAP concentration ranges in pregnant women in Japan. Methods Urine samples were collected from 62 non-smoking pregnant women (12–22 weeks of gestation) living in Aichi Prefecture, Japan. First morning void (FMV) and spot urine samples taken between lunch and dinner on the same day were collected on five different days during 2 weeks. The concentrations of DAP and creatinine in urine samples were measured using an ultra performance liquid chromatography with tandem mass spectrometry. Creatinine-adjusted and unadjusted concentrations were used for the intraclass correlation coefficient (ICC) calculations and surrogate category analyses. Results For all DAP metabolites, the creatinine-adjusted single ICCs exceeded 0.4, indicating moderate reliability. Overall, ICCs of spot urine samples taken in the afternoon were better than those taken as FMV. Surrogate category analyses showed that participants were categorized accurately into four exposure dose groups according to the quartile points. Conclusion This study indicated that a single urine sample taken in the afternoon may be useful in assessing OP exposure as long as the exposure is categorized into quartiles when conducting epidemiological studies in early to mid-pregnant women in Japan. |
| first_indexed | 2024-04-13T19:45:26Z |
| format | Article |
| id | doaj.art-8acbc8753a96424087fc0e029f041440 |
| institution | Directory Open Access Journal |
| issn | 1342-078X 1347-4715 |
| language | English |
| last_indexed | 2024-04-13T19:45:26Z |
| publishDate | 2019-01-01 |
| publisher | Komiyama Printing Co. Ltd |
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| series | Environmental Health and Preventive Medicine |
| spelling | doaj.art-8acbc8753a96424087fc0e029f0414402022-12-22T02:32:45ZengKomiyama Printing Co. LtdEnvironmental Health and Preventive Medicine1342-078X1347-47152019-01-012411610.1186/s12199-019-0761-4Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in JapanKeisuke Hioki0Yuki Ito1Naoko Oya2Shoji F. Nakayama3Tomohiko Isobe4Takeshi Ebara5Kanemitsu Shibata6Naomi Nishikawa7Kunihiko Nakai8Tomota Kamida9Jun Ueyama10Mayumi Sugiura-Ogasawara11Michihiro Kamijima12Department of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesDepartment of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesDepartment of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesCenter for Health and Environmental Risk Research, National Institute for Environmental StudiesCenter for Health and Environmental Risk Research, National Institute for Environmental StudiesDepartment of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesDepartment of Obstetrics and Gynecology, Nagoya City West Medical CenterDepartment of Obstetrics and Gynecology, Nagoya City West Medical CenterDepartment of Development and Environmental Medicine, Tohoku University Graduate School of MedicineDepartment of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesDepartment of Medical Technology, Nagoya University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Nagoya City University Graduate School of Medical SciencesDepartment of Occupational and Environmental Health, Nagoya City University Graduate School of Medical SciencesAbstract Background Low-dose exposure to organophosphate (OP) insecticides during pregnancy may adversely affect neurodevelopment in children. To evaluate the OP exposure levels, single urine sampling is commonly adopted to measure the levels of dialkylphosphates (DAPs), common OP metabolites. However, the inter-day variations of urinary DAP concentrations within subjects are supposed to be large due to the short biological half-lives of the metabolites, and it is thus considered difficult to accurately assess OP exposure during pregnancy with single sampling. This study aimed to assess intra-individual variations of DAP concentrations and the reproducibility of the exposure dose categorization of OPs according to DAP concentration ranges in pregnant women in Japan. Methods Urine samples were collected from 62 non-smoking pregnant women (12–22 weeks of gestation) living in Aichi Prefecture, Japan. First morning void (FMV) and spot urine samples taken between lunch and dinner on the same day were collected on five different days during 2 weeks. The concentrations of DAP and creatinine in urine samples were measured using an ultra performance liquid chromatography with tandem mass spectrometry. Creatinine-adjusted and unadjusted concentrations were used for the intraclass correlation coefficient (ICC) calculations and surrogate category analyses. Results For all DAP metabolites, the creatinine-adjusted single ICCs exceeded 0.4, indicating moderate reliability. Overall, ICCs of spot urine samples taken in the afternoon were better than those taken as FMV. Surrogate category analyses showed that participants were categorized accurately into four exposure dose groups according to the quartile points. Conclusion This study indicated that a single urine sample taken in the afternoon may be useful in assessing OP exposure as long as the exposure is categorized into quartiles when conducting epidemiological studies in early to mid-pregnant women in Japan.http://link.springer.com/article/10.1186/s12199-019-0761-4Organophosphate insecticidesPregnant womenIntraclass correlation coefficientsReproducibility |
| spellingShingle | Keisuke Hioki Yuki Ito Naoko Oya Shoji F. Nakayama Tomohiko Isobe Takeshi Ebara Kanemitsu Shibata Naomi Nishikawa Kunihiko Nakai Tomota Kamida Jun Ueyama Mayumi Sugiura-Ogasawara Michihiro Kamijima Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan Environmental Health and Preventive Medicine Organophosphate insecticides Pregnant women Intraclass correlation coefficients Reproducibility |
| title | Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan |
| title_full | Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan |
| title_fullStr | Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan |
| title_full_unstemmed | Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan |
| title_short | Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan |
| title_sort | intra individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in japan |
| topic | Organophosphate insecticides Pregnant women Intraclass correlation coefficients Reproducibility |
| url | http://link.springer.com/article/10.1186/s12199-019-0761-4 |
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