Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection
Purpose To compare the adaptation processes of the remaining jejunum or ileum after extensive intestinal resection in a growing animal model. Materials and Methods Forty 21-day old rats were divided into four groups: JG: remaining jejunum group – ileal enterectomy; IG: ileum remaining group – jejunu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-04-01
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| Series: | Journal of Investigative Surgery |
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| Online Access: | http://dx.doi.org/10.1080/08941939.2021.1963355 |
| _version_ | 1797684481722679296 |
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| author | Affonso Flávio Jorge Mussolino Ana Cristina Aoun Tannuri Josiane de Oliveira Gonçalves Suellen Serafini Uenis Tannuri |
| author_facet | Affonso Flávio Jorge Mussolino Ana Cristina Aoun Tannuri Josiane de Oliveira Gonçalves Suellen Serafini Uenis Tannuri |
| author_sort | Affonso Flávio Jorge Mussolino |
| collection | DOAJ |
| description | Purpose To compare the adaptation processes of the remaining jejunum or ileum after extensive intestinal resection in a growing animal model. Materials and Methods Forty 21-day old rats were divided into four groups: JG: remaining jejunum group – ileal enterectomy; IG: ileum remaining group – jejunum enterectomy; SHAM: sham-operated group – open-and-close laparotomy; and NO: non-operated animals. Results After 3 weeks, JG and IG animals had decreased weights comparing to SHAM and NO animals (p = 0.017 and p = 0.005, respectively). The histomorphometric analysis showed that in JG animals the villi were higher than in SHAM, NO, and IG animals (p = 0.007, p = 0.008, and p = 0.01), the depth of crypts in JG and IG animals was greater than in NO and SHAM animals (p = 0.03, p = 0.002, and p = 0.003 respectively), and muscle layer thickness of the jejunum of JG animals had values greater than SHAM and NO animals (p = 0.01 and p = 0.02, respectively). The Ki-67 expression in the ileum was higher in comparison with the jejunum (p = 0.014). The pro-apoptotic gene (Bax) expression was decreased in JG animals compared to IG, SHAM, and NO animals (p = 0.013, p = 0.024, and p = 0.021). The anti-apoptotic gene (Bcl-XL) expression was decreased in JG animals in comparison to IG and NO animals (p = 0.002 and p = 0.046) although it was increased in the colon of IG animals in comparison to JG, SHAM, and NO animals (p = 0.002, p = 0.001, and p = 0.001, respectively). The Bax/Bcl-XL ratio was higher in JG than in IG animals (p = 0.011). Conclusion Adaptive responses seemed to be more effective in the ileum than in the jejunum. |
| first_indexed | 2024-03-12T00:30:19Z |
| format | Article |
| id | doaj.art-8acc26192aee4b5a9e5c21e5ff688f87 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:30:19Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-8acc26192aee4b5a9e5c21e5ff688f872023-09-15T10:21:28ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532022-04-0135479380010.1080/08941939.2021.19633551963355Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal ResectionAffonso Flávio Jorge Mussolino0Ana Cristina Aoun Tannuri1Josiane de Oliveira Gonçalves2Suellen Serafini3Uenis Tannuri4Pediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical SchoolPediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical SchoolPediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical SchoolPediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical SchoolPediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical SchoolPurpose To compare the adaptation processes of the remaining jejunum or ileum after extensive intestinal resection in a growing animal model. Materials and Methods Forty 21-day old rats were divided into four groups: JG: remaining jejunum group – ileal enterectomy; IG: ileum remaining group – jejunum enterectomy; SHAM: sham-operated group – open-and-close laparotomy; and NO: non-operated animals. Results After 3 weeks, JG and IG animals had decreased weights comparing to SHAM and NO animals (p = 0.017 and p = 0.005, respectively). The histomorphometric analysis showed that in JG animals the villi were higher than in SHAM, NO, and IG animals (p = 0.007, p = 0.008, and p = 0.01), the depth of crypts in JG and IG animals was greater than in NO and SHAM animals (p = 0.03, p = 0.002, and p = 0.003 respectively), and muscle layer thickness of the jejunum of JG animals had values greater than SHAM and NO animals (p = 0.01 and p = 0.02, respectively). The Ki-67 expression in the ileum was higher in comparison with the jejunum (p = 0.014). The pro-apoptotic gene (Bax) expression was decreased in JG animals compared to IG, SHAM, and NO animals (p = 0.013, p = 0.024, and p = 0.021). The anti-apoptotic gene (Bcl-XL) expression was decreased in JG animals in comparison to IG and NO animals (p = 0.002 and p = 0.046) although it was increased in the colon of IG animals in comparison to JG, SHAM, and NO animals (p = 0.002, p = 0.001, and p = 0.001, respectively). The Bax/Bcl-XL ratio was higher in JG than in IG animals (p = 0.011). Conclusion Adaptive responses seemed to be more effective in the ileum than in the jejunum.http://dx.doi.org/10.1080/08941939.2021.1963355animal modelexperimental surgerypediatric surgeryintestinegastroenterologyabdominal surgery |
| spellingShingle | Affonso Flávio Jorge Mussolino Ana Cristina Aoun Tannuri Josiane de Oliveira Gonçalves Suellen Serafini Uenis Tannuri Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection Journal of Investigative Surgery animal model experimental surgery pediatric surgery intestine gastroenterology abdominal surgery |
| title | Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection |
| title_full | Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection |
| title_fullStr | Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection |
| title_full_unstemmed | Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection |
| title_short | Adaptation Processes of the Remaining Jejunum or Ileum after Extensive Intestinal Resection |
| title_sort | adaptation processes of the remaining jejunum or ileum after extensive intestinal resection |
| topic | animal model experimental surgery pediatric surgery intestine gastroenterology abdominal surgery |
| url | http://dx.doi.org/10.1080/08941939.2021.1963355 |
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