Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L.
The objective of this study was to evaluate antifungal and antioxidant activities of Hypericum perforatum L. extracts against the growth of certain fungi. The ethanolic, acetonic and petroleum ether extracts of the plant were evaluated for phenols, flavonoids and non-flavonoids. The highest amoun...
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| Format: | Article |
| Language: | English |
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Association of Chemical Engineers of Serbia
2011-01-01
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| Series: | Hemijska Industrija |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100004M.pdf |
| _version_ | 1811251049205334016 |
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| author | Mašković Pavle Z. Mladenović Jelena D. Cvijović Milica S. Aćamović-Đoković Gordana Solujić Slavica R. Radojković Marija M. |
| author_facet | Mašković Pavle Z. Mladenović Jelena D. Cvijović Milica S. Aćamović-Đoković Gordana Solujić Slavica R. Radojković Marija M. |
| author_sort | Mašković Pavle Z. |
| collection | DOAJ |
| description | The objective of this study was to evaluate antifungal and antioxidant activities of Hypericum perforatum L. extracts against the growth of certain fungi. The ethanolic, acetonic and petroleum ether extracts of the plant were evaluated for phenols, flavonoids and non-flavonoids. The highest amounts of phenols (17.6 mg EPC/g dry extract) and flavonoids (16.85 mg EPC/g dry extract) were found in the acetonic extract. The highest inhibitory effect on the growth of Penicillium canescens, Fusarium oxysporum, Alternaria alternata, Aspergillus glaucus and Phialophora fastigiata by the disk diffusion method was exhibited by the ethanolic extract at the concentration of 25 mg/disk. The minimum inhibitory concentration (MIC) of the ethanolic and petroleum ether extracts was 20 mg/mL. The acetonic extract did not affect the growth of the tested fungi. Antioxidant activity was assessed by determining 1,1-Diphenyl-2-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity. The results showed that the ethanolic extract of Hypericum perforatum L. possesses antioxidant activity. The IC50 values, defined as the concentration of the test sample leading to 50% reduction of the free radical concentration, determined for each measurement were <7.8125, 105.9, 5.99 and 12.77 μg/ml for the ethanolic extract, the acetonic extract, ascorbic acid and BHT, respectively, for DPPH free radical scavenging activity. |
| first_indexed | 2024-04-12T16:14:43Z |
| format | Article |
| id | doaj.art-8ad172f27691480d8be9f8034c8a3a67 |
| institution | Directory Open Access Journal |
| issn | 0367-598X |
| language | English |
| last_indexed | 2024-04-12T16:14:43Z |
| publishDate | 2011-01-01 |
| publisher | Association of Chemical Engineers of Serbia |
| record_format | Article |
| series | Hemijska Industrija |
| spelling | doaj.art-8ad172f27691480d8be9f8034c8a3a672022-12-22T03:25:47ZengAssociation of Chemical Engineers of SerbiaHemijska Industrija0367-598X2011-01-0165215916410.2298/HEMIND100819004MPhenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L.Mašković Pavle Z.Mladenović Jelena D.Cvijović Milica S.Aćamović-Đoković GordanaSolujić Slavica R.Radojković Marija M.The objective of this study was to evaluate antifungal and antioxidant activities of Hypericum perforatum L. extracts against the growth of certain fungi. The ethanolic, acetonic and petroleum ether extracts of the plant were evaluated for phenols, flavonoids and non-flavonoids. The highest amounts of phenols (17.6 mg EPC/g dry extract) and flavonoids (16.85 mg EPC/g dry extract) were found in the acetonic extract. The highest inhibitory effect on the growth of Penicillium canescens, Fusarium oxysporum, Alternaria alternata, Aspergillus glaucus and Phialophora fastigiata by the disk diffusion method was exhibited by the ethanolic extract at the concentration of 25 mg/disk. The minimum inhibitory concentration (MIC) of the ethanolic and petroleum ether extracts was 20 mg/mL. The acetonic extract did not affect the growth of the tested fungi. Antioxidant activity was assessed by determining 1,1-Diphenyl-2-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity. The results showed that the ethanolic extract of Hypericum perforatum L. possesses antioxidant activity. The IC50 values, defined as the concentration of the test sample leading to 50% reduction of the free radical concentration, determined for each measurement were <7.8125, 105.9, 5.99 and 12.77 μg/ml for the ethanolic extract, the acetonic extract, ascorbic acid and BHT, respectively, for DPPH free radical scavenging activity.http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100004M.pdfantifungal activityHypericum perforatumtotal phenolsantioxidant |
| spellingShingle | Mašković Pavle Z. Mladenović Jelena D. Cvijović Milica S. Aćamović-Đoković Gordana Solujić Slavica R. Radojković Marija M. Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. Hemijska Industrija antifungal activity Hypericum perforatum total phenols antioxidant |
| title | Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. |
| title_full | Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. |
| title_fullStr | Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. |
| title_full_unstemmed | Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. |
| title_short | Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L. |
| title_sort | phenolic content antioxidant and antifungal activities of acetonic ethanolic and petroleum ether extracts of hypericum perforatum l |
| topic | antifungal activity Hypericum perforatum total phenols antioxidant |
| url | http://www.doiserbia.nb.rs/img/doi/0367-598X/2011/0367-598X1100004M.pdf |
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