FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PC...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
|
| Series: | Adipocyte |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21623945.2021.1986327 |
| _version_ | 1818966925116440576 |
|---|---|
| author | Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan |
| author_facet | Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan |
| author_sort | Chengcheng Liang |
| collection | DOAJ |
| description | The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation. |
| first_indexed | 2024-12-20T13:40:38Z |
| format | Article |
| id | doaj.art-8ad2ecac595844b58e8c9703e6ec8a32 |
| institution | Directory Open Access Journal |
| issn | 2162-3945 2162-397X |
| language | English |
| last_indexed | 2024-12-20T13:40:38Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj.art-8ad2ecac595844b58e8c9703e6ec8a322022-12-21T19:38:50ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2021-01-0110154655710.1080/21623945.2021.19863271986327FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathwayChengcheng Liang0Guohua Wang1Sayed Haidar Abbas Raza2Xiaoyu Wang3Bingzhi Li4Wenzhen Zhang5Linsen Zan6Northwest A&f UniversityNorthwest A&f UniversityNorthwest A&f UniversityNorthwest A&f UniversityNorthwest A&f UniversityNorthwest A&f UniversityNorthwest A&f UniversityThe family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.http://dx.doi.org/10.1080/21623945.2021.1986327fam13ahypoxiaproliferationhif-1 pathway |
| spellingShingle | Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway Adipocyte fam13a hypoxia proliferation hif-1 pathway |
| title | FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway |
| title_full | FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway |
| title_fullStr | FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway |
| title_full_unstemmed | FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway |
| title_short | FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway |
| title_sort | fam13a promotes proliferation of bovine preadipocytes by targeting hypoxia inducible factor 1 signaling pathway |
| topic | fam13a hypoxia proliferation hif-1 pathway |
| url | http://dx.doi.org/10.1080/21623945.2021.1986327 |
| work_keys_str_mv | AT chengchengliang fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT guohuawang fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT sayedhaidarabbasraza fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT xiaoyuwang fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT bingzhili fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT wenzhenzhang fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway AT linsenzan fam13apromotesproliferationofbovinepreadipocytesbytargetinghypoxiainduciblefactor1signalingpathway |