The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2
This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-04-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/5/2666 |
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| author | Ji Sook Kang Il-Whan Choi Min Ho Han Dae-Sung Lee Gi-Young Kim Hye Jin Hwang Byung Woo Kim Cheol Min Kim Young Hyun Yoo Yung Hyun Choi |
| author_facet | Ji Sook Kang Il-Whan Choi Min Ho Han Dae-Sung Lee Gi-Young Kim Hye Jin Hwang Byung Woo Kim Cheol Min Kim Young Hyun Yoo Yung Hyun Choi |
| author_sort | Ji Sook Kang |
| collection | DOAJ |
| description | This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway. |
| first_indexed | 2024-04-11T17:57:42Z |
| format | Article |
| id | doaj.art-8ad37bb6ffcb40ce8b692a1046a85cc1 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T17:57:42Z |
| publishDate | 2015-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8ad37bb6ffcb40ce8b692a1046a85cc12022-12-22T04:10:36ZengMDPI AGMarine Drugs1660-33972015-04-011352666267910.3390/md13052666md13052666The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2Ji Sook Kang0Il-Whan Choi1Min Ho Han2Dae-Sung Lee3Gi-Young Kim4Hye Jin Hwang5Byung Woo Kim6Cheol Min Kim7Young Hyun Yoo8Yung Hyun Choi9Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, KoreaDepartment of Microbiology, College of Medicine, Inje University, Busan 608-756, KoreaMarine Biodiversity Institute of Korea, Seocheon 325-902, KoreaMarine Biodiversity Institute of Korea, Seocheon 325-902, KoreaLaboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, KoreaBlue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, KoreaBlue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, KoreaDepartment of Biochemistry, Busan National University College of Medicine, Yangsan 626-870, KoreaDepartment of Anatomy and Cell Biology, Dong-A University College of Medicine & Mitochondria Hub Regulation Center, Busan 602-714, KoreaBlue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, KoreaThis study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway.http://www.mdpi.com/1660-3397/13/5/2666Petalonia binghamiaeoxidative stressROSDNA damageNrf2/HO-1 |
| spellingShingle | Ji Sook Kang Il-Whan Choi Min Ho Han Dae-Sung Lee Gi-Young Kim Hye Jin Hwang Byung Woo Kim Cheol Min Kim Young Hyun Yoo Yung Hyun Choi The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 Marine Drugs Petalonia binghamiae oxidative stress ROS DNA damage Nrf2/HO-1 |
| title | The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 |
| title_full | The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 |
| title_fullStr | The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 |
| title_full_unstemmed | The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 |
| title_short | The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2 |
| title_sort | cytoprotective effect of petalonia binghamiae methanol extract against oxidative stress in c2c12 myoblasts mediation by upregulation of heme oxygenase 1 and nuclear factor erythroid 2 related factor 2 |
| topic | Petalonia binghamiae oxidative stress ROS DNA damage Nrf2/HO-1 |
| url | http://www.mdpi.com/1660-3397/13/5/2666 |
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