Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity
Lingyun pekoe as starting material, studies were carried out to optimize the extracting technology of polysaccharides, and investigate its antioxidant activity. Extracting yield of polysaccharides as the index, the extraction conditions were optimized by Box-Bohnken method based on the results of si...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-05-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021080117 |
| _version_ | 1811224603073183744 |
|---|---|
| author | Liqing XIAN Shan LI Bin FENG Jian LIANG Xiaofeng LIU |
| author_facet | Liqing XIAN Shan LI Bin FENG Jian LIANG Xiaofeng LIU |
| author_sort | Liqing XIAN |
| collection | DOAJ |
| description | Lingyun pekoe as starting material, studies were carried out to optimize the extracting technology of polysaccharides, and investigate its antioxidant activity. Extracting yield of polysaccharides as the index, the extraction conditions were optimized by Box-Bohnken method based on the results of single-factor experiments. The antioxidant activities were evaluated by the determination of hydroxyl radical (·OH), 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH·) , and inhibition effect on auto-oxidation of grease. Experimental results showed that, the optimal extraction conditions were obtained as follows: liquid-material ratio 69:1 mL/g, temperature 52 ℃, ultrasonic time 21 min, ultrasonic power 180 W. Under the optimal conditions, an average extraction yield (4.84%±0.04%) was obtained, which was similar with the expected yield 5.06% (<5%) of regressive equation. The IC50 of polysaccharides for eliminating ·OH, DPPH· were 0.262, 0.438 mg/mL, and a better clearance result would be obtained with higher concentration. Moreover, polysaccharides could prolong the storage time of grease in normal circumstance for inhibiting the auto-oxidation. The studies mentioned above indicated that polysaccharides were plentiful in Lingyun poeke, and had a good antioxidant activity, providing data support for promoting in home and abroad. |
| first_indexed | 2024-04-12T08:51:50Z |
| format | Article |
| id | doaj.art-8ad82c9719c54a1d9c872389a486e634 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:51:50Z |
| publishDate | 2022-05-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-8ad82c9719c54a1d9c872389a486e6342022-12-22T03:39:33ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-05-0143917818410.13386/j.issn1002-0306.20210801172021080117-9Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant ActivityLiqing XIAN0Shan LI1Bin FENG2Jian LIANG3Xiaofeng LIU4Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, ChinaKey Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, ChinaKey Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, ChinaKey Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, ChinaKey Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, ChinaLingyun pekoe as starting material, studies were carried out to optimize the extracting technology of polysaccharides, and investigate its antioxidant activity. Extracting yield of polysaccharides as the index, the extraction conditions were optimized by Box-Bohnken method based on the results of single-factor experiments. The antioxidant activities were evaluated by the determination of hydroxyl radical (·OH), 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH·) , and inhibition effect on auto-oxidation of grease. Experimental results showed that, the optimal extraction conditions were obtained as follows: liquid-material ratio 69:1 mL/g, temperature 52 ℃, ultrasonic time 21 min, ultrasonic power 180 W. Under the optimal conditions, an average extraction yield (4.84%±0.04%) was obtained, which was similar with the expected yield 5.06% (<5%) of regressive equation. The IC50 of polysaccharides for eliminating ·OH, DPPH· were 0.262, 0.438 mg/mL, and a better clearance result would be obtained with higher concentration. Moreover, polysaccharides could prolong the storage time of grease in normal circumstance for inhibiting the auto-oxidation. The studies mentioned above indicated that polysaccharides were plentiful in Lingyun poeke, and had a good antioxidant activity, providing data support for promoting in home and abroad.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021080117lingyun pekoepolysaccharidesultrasonic-assisted extractionbox-bohnken methodantioxidant activity |
| spellingShingle | Liqing XIAN Shan LI Bin FENG Jian LIANG Xiaofeng LIU Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity Shipin gongye ke-ji lingyun pekoe polysaccharides ultrasonic-assisted extraction box-bohnken method antioxidant activity |
| title | Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity |
| title_full | Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity |
| title_fullStr | Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity |
| title_full_unstemmed | Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity |
| title_short | Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity |
| title_sort | optimization of ultrasonic extraction of polysaccharides in lingyun pekoe and its antioxidant activity |
| topic | lingyun pekoe polysaccharides ultrasonic-assisted extraction box-bohnken method antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021080117 |
| work_keys_str_mv | AT liqingxian optimizationofultrasonicextractionofpolysaccharidesinlingyunpekoeanditsantioxidantactivity AT shanli optimizationofultrasonicextractionofpolysaccharidesinlingyunpekoeanditsantioxidantactivity AT binfeng optimizationofultrasonicextractionofpolysaccharidesinlingyunpekoeanditsantioxidantactivity AT jianliang optimizationofultrasonicextractionofpolysaccharidesinlingyunpekoeanditsantioxidantactivity AT xiaofengliu optimizationofultrasonicextractionofpolysaccharidesinlingyunpekoeanditsantioxidantactivity |