Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve
<p class="00Testo">Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of elastin and collagen fibers. Alterations of extracellular matrix structure and protein composition, typical...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2014-07-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2383 |
| _version_ | 1818119570874957824 |
|---|---|
| author | A. Arcucci M.R. Ruocco F. Albano G. Granato V. Romano G. Corso C. Bancone E. De Vendittis A. Della Corte S. Montagnani |
| author_facet | A. Arcucci M.R. Ruocco F. Albano G. Granato V. Romano G. Corso C. Bancone E. De Vendittis A. Della Corte S. Montagnani |
| author_sort | A. Arcucci |
| collection | DOAJ |
| description | <p class="00Testo">Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of elastin and collagen fibers. Alterations of extracellular matrix structure and protein composition, typical of medial degeneration, can modulate intracellular pathways. In this study we examined the relevance of superoxide dismutase (SOD3) and Akt in AsAA pathogenesis, evaluating their tissue distribution and protein levels in ascending aortic tissues from controls (n=6), patients affected by AsAA associated to tricuspid aortic valve (TAV, n=9) or bicuspid aortic valve <strong>(</strong>BAV, n=9). The results showed a significant reduction of SOD3, phospho-Akt and Akt protein levels in AsAA tissues from patients with BAV, compared to controls, whereas the differences observed between controls and patients with TAV were not significant. The decreased levels of SOD3 and Akt in BAV aortic tissues are associated with decreased Erk1/Erk2 phosphorylation and MMP-9 levels increase. The authors suggest a role of decreased SOD3 protein levels in the progression of AsAA with BAV and a link between ECM modifications of aortic media layer and impaired Erk1/Erk2 and Akt<strong><em> </em></strong>signaling in the late stages of the aortopathy associated with BAV.</p> |
| first_indexed | 2024-12-11T05:12:18Z |
| format | Article |
| id | doaj.art-8adba09aafcb494e8d9ca762398134b3 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T05:12:18Z |
| publishDate | 2014-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-8adba09aafcb494e8d9ca762398134b32022-12-22T01:19:54ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062014-07-0158310.4081/ejh.2014.23831410Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valveA. Arcucci0M.R. Ruocco1F. Albano2G. Granato3V. Romano4G. Corso5C. Bancone6E. De Vendittis7A. Della Corte8S. Montagnani9University of Naples Federico IIUniversity of Naples Federico IIUniversity of Naples Federico IIUniversity of Naples Federico IIUniversity of Naples Federico IIUniversity of FoggiaSecond University of NaplesUniversity of Naples Federico IISecond University of NaplesUniversity of Naples Federico II<p class="00Testo">Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of elastin and collagen fibers. Alterations of extracellular matrix structure and protein composition, typical of medial degeneration, can modulate intracellular pathways. In this study we examined the relevance of superoxide dismutase (SOD3) and Akt in AsAA pathogenesis, evaluating their tissue distribution and protein levels in ascending aortic tissues from controls (n=6), patients affected by AsAA associated to tricuspid aortic valve (TAV, n=9) or bicuspid aortic valve <strong>(</strong>BAV, n=9). The results showed a significant reduction of SOD3, phospho-Akt and Akt protein levels in AsAA tissues from patients with BAV, compared to controls, whereas the differences observed between controls and patients with TAV were not significant. The decreased levels of SOD3 and Akt in BAV aortic tissues are associated with decreased Erk1/Erk2 phosphorylation and MMP-9 levels increase. The authors suggest a role of decreased SOD3 protein levels in the progression of AsAA with BAV and a link between ECM modifications of aortic media layer and impaired Erk1/Erk2 and Akt<strong><em> </em></strong>signaling in the late stages of the aortopathy associated with BAV.</p>http://www.ejh.it/index.php/ejh/article/view/2383Ascending aortic aneurysmbicuspid aortic valvemedial degenerationsmooth muscle cellsextracellular superoxide dismutaseAkttricuspid aortic valve. |
| spellingShingle | A. Arcucci M.R. Ruocco F. Albano G. Granato V. Romano G. Corso C. Bancone E. De Vendittis A. Della Corte S. Montagnani Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve European Journal of Histochemistry Ascending aortic aneurysm bicuspid aortic valve medial degeneration smooth muscle cells extracellular superoxide dismutase Akt tricuspid aortic valve. |
| title | Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| title_full | Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| title_fullStr | Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| title_full_unstemmed | Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| title_short | Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| title_sort | analysis of extracellular superoxide dismutase and akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve |
| topic | Ascending aortic aneurysm bicuspid aortic valve medial degeneration smooth muscle cells extracellular superoxide dismutase Akt tricuspid aortic valve. |
| url | http://www.ejh.it/index.php/ejh/article/view/2383 |
| work_keys_str_mv | AT aarcucci analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT mrruocco analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT falbano analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT ggranato analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT vromano analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT gcorso analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT cbancone analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT edevendittis analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT adellacorte analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve AT smontagnani analysisofextracellularsuperoxidedismutaseandaktinascendingaorticaneurysmwithtricuspidorbicuspidaorticvalve |