Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection
This work aimed to fabricate an optical urea biosensor using anthocyanins extracted from the Catharanthus roseus L as pH-sensitive substances and immobilised on a polyelectrolyte complex (PEC) pectin-chitosan membrane. The PEC pectin-chitosan/anthocyanin system's first layer is an optical pH se...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715624000365 |
| _version_ | 1797305303886200832 |
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| author | Eka Safitri Nazaruddin Nazaruddin Nurhayati Teuku Aldiansyah Wan Elina Faradilla Wan Khalid Salsabilla Latansa Nazaruddin Muhammad Bahi Muhammad Iqhrammullah |
| author_facet | Eka Safitri Nazaruddin Nazaruddin Nurhayati Teuku Aldiansyah Wan Elina Faradilla Wan Khalid Salsabilla Latansa Nazaruddin Muhammad Bahi Muhammad Iqhrammullah |
| author_sort | Eka Safitri |
| collection | DOAJ |
| description | This work aimed to fabricate an optical urea biosensor using anthocyanins extracted from the Catharanthus roseus L as pH-sensitive substances and immobilised on a polyelectrolyte complex (PEC) pectin-chitosan membrane. The PEC pectin-chitosan/anthocyanin system's first layer is an optical pH sensor optimised in previous research. Then, the optimised pH sensor was further modified as an optical urea biosensor by immobilising the urease as the second layer. The biosensor relied on pH changes resulting from the reaction between urease and urea. The best sensitivity was observed at 0.09 mg urease, the optimum response was obtained at 0.03 M phosphate buffer solution of pH 7, and the stability was achieved after 5 min of measurement. The sensitivity reached at the widest dynamic range of urea concentration (1 × 10−10 to 1 × 10−1 M) was 0.0218 with a detection limit of 5.2 × 10−12 M. The optimised urea biosensor has repeatability with a percentage relative standard deviation of 10.2 %. The lifetime was evaluated for 30 days of measurements, where fluctuation in sensor sensitivity was observed. |
| first_indexed | 2024-03-08T00:23:57Z |
| format | Article |
| id | doaj.art-8adc04c2fa5d494f82d5f9b058c66b65 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-08T00:23:57Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-8adc04c2fa5d494f82d5f9b058c66b652024-02-16T04:28:59ZengElsevierResults in Chemistry2211-71562024-01-017101340Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detectionEka Safitri0Nazaruddin Nazaruddin1 Nurhayati2Teuku Aldiansyah3Wan Elina Faradilla Wan Khalid4Salsabilla Latansa Nazaruddin5Muhammad Bahi6Muhammad Iqhrammullah7Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam 23111, Banda Aceh, Indonesia; Corresponding author.Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam 23111, Banda Aceh, IndonesiaPoliteknik Pelayaran Malahayati, Badan Pengembangan Sumber Daya Manusia Perhubungan, Kementrian Perhubungan, Aceh Besar 23381, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam 23111, Banda Aceh, IndonesiaFaculty of Applied Sciences, UiTM Cawangan Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi, 72000 Kuala Pilah, Negeri Sembilan, MalaysiaFaculty of Dentistry, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam 23111, Banda Aceh, IndonesiaResearch Center for Marine and Land Bioindustry, National Research and Innovation Agency (BRIN), North Lombok 83756, IndonesiaThis work aimed to fabricate an optical urea biosensor using anthocyanins extracted from the Catharanthus roseus L as pH-sensitive substances and immobilised on a polyelectrolyte complex (PEC) pectin-chitosan membrane. The PEC pectin-chitosan/anthocyanin system's first layer is an optical pH sensor optimised in previous research. Then, the optimised pH sensor was further modified as an optical urea biosensor by immobilising the urease as the second layer. The biosensor relied on pH changes resulting from the reaction between urease and urea. The best sensitivity was observed at 0.09 mg urease, the optimum response was obtained at 0.03 M phosphate buffer solution of pH 7, and the stability was achieved after 5 min of measurement. The sensitivity reached at the widest dynamic range of urea concentration (1 × 10−10 to 1 × 10−1 M) was 0.0218 with a detection limit of 5.2 × 10−12 M. The optimised urea biosensor has repeatability with a percentage relative standard deviation of 10.2 %. The lifetime was evaluated for 30 days of measurements, where fluctuation in sensor sensitivity was observed.http://www.sciencedirect.com/science/article/pii/S2211715624000365UreaBiosensorPEC pectin-chitosanCatharanthus roseus L |
| spellingShingle | Eka Safitri Nazaruddin Nazaruddin Nurhayati Teuku Aldiansyah Wan Elina Faradilla Wan Khalid Salsabilla Latansa Nazaruddin Muhammad Bahi Muhammad Iqhrammullah Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection Results in Chemistry Urea Biosensor PEC pectin-chitosan Catharanthus roseus L |
| title | Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection |
| title_full | Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection |
| title_fullStr | Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection |
| title_full_unstemmed | Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection |
| title_short | Optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and anthocyanin from Catharanthus roseus L for a salivary simple urea detection |
| title_sort | optical urea biosensor based on polyelectrolyte complex pec pectin chitosan membrane and anthocyanin from catharanthus roseus l for a salivary simple urea detection |
| topic | Urea Biosensor PEC pectin-chitosan Catharanthus roseus L |
| url | http://www.sciencedirect.com/science/article/pii/S2211715624000365 |
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