Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran
Background: The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species. Objective: The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid co...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2012-08-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-141-en.html |
| _version_ | 1818664357407490048 |
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| author | F Khalighi-Sigaroodi M Ahvazi D Yazdani M Kashefi |
| author_facet | F Khalighi-Sigaroodi M Ahvazi D Yazdani M Kashefi |
| author_sort | F Khalighi-Sigaroodi |
| collection | DOAJ |
| description | Background: The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species.
Objective: The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid contents of five plant species of Solanaceae family.
Methods: Five plant species of Solanaceae family were collected from different regions of Iran. Methanol extracts and chloroform fractions of these species were tested by brine shrimp lethality assay in order to detect cytotoxicity. Antioxidant activity was evaluated by DPPH method. The total phenol content was measured using Folin - Ciocalteu method. The flavonoid content was measured by a colorimetric assay.
Results: The extracts of Datura innoxia and Datura stramonium showed the highest cytotoxicity activities with LC50 values of 22.08 and 21.66 μg/ml, respectively. The chloroform fractions of these two species were subjected to cytotoxicity assay with LC50 values of 33.00 and 4.29 μg/ml, respectively. In comparing, Solanum dulcamara showed the highest antioxidant activity with IC50 values of 52.51 μg/ml and the highest phenol and flavonoid content of the dry weight.
Conclusion: It could be seen among five tested plant species that D. stramonium had the highest cytotoxic activity and S. dulcamara had the highest antioxidant activity, phenol and flavonoid content. Further studies are necessary for chemical composition of the extracts and more comprehensive biological assays. |
| first_indexed | 2024-12-17T05:31:27Z |
| format | Article |
| id | doaj.art-8ade6109331b445c958e43234a2d1397 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-17T05:31:27Z |
| publishDate | 2012-08-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-8ade6109331b445c958e43234a2d13972022-12-21T22:01:43ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582012-08-0111434153Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from IranF Khalighi-Sigaroodi0M Ahvazi1D Yazdani2M Kashefi3 Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Background: The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species. Objective: The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid contents of five plant species of Solanaceae family. Methods: Five plant species of Solanaceae family were collected from different regions of Iran. Methanol extracts and chloroform fractions of these species were tested by brine shrimp lethality assay in order to detect cytotoxicity. Antioxidant activity was evaluated by DPPH method. The total phenol content was measured using Folin - Ciocalteu method. The flavonoid content was measured by a colorimetric assay. Results: The extracts of Datura innoxia and Datura stramonium showed the highest cytotoxicity activities with LC50 values of 22.08 and 21.66 μg/ml, respectively. The chloroform fractions of these two species were subjected to cytotoxicity assay with LC50 values of 33.00 and 4.29 μg/ml, respectively. In comparing, Solanum dulcamara showed the highest antioxidant activity with IC50 values of 52.51 μg/ml and the highest phenol and flavonoid content of the dry weight. Conclusion: It could be seen among five tested plant species that D. stramonium had the highest cytotoxic activity and S. dulcamara had the highest antioxidant activity, phenol and flavonoid content. Further studies are necessary for chemical composition of the extracts and more comprehensive biological assays.http://jmp.ir/article-1-141-en.htmlantioxidantcytotoxicityflavonoidphenolsolanaceae |
| spellingShingle | F Khalighi-Sigaroodi M Ahvazi D Yazdani M Kashefi Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran Journal of Medicinal Plants antioxidant cytotoxicity flavonoid phenol solanaceae |
| title | Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran |
| title_full | Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran |
| title_fullStr | Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran |
| title_full_unstemmed | Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran |
| title_short | Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran |
| title_sort | cytotoxicity and antioxidant activity of five plant species of solanaceae family from iran |
| topic | antioxidant cytotoxicity flavonoid phenol solanaceae |
| url | http://jmp.ir/article-1-141-en.html |
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