Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot
Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its co...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-09-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2023-034 |
| _version_ | 1797674845767467008 |
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| author | Li Si Zhang Fu-Mei Shang Xiao-Jing Hou Rui |
| author_facet | Li Si Zhang Fu-Mei Shang Xiao-Jing Hou Rui |
| author_sort | Li Si |
| collection | DOAJ |
| description | Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot. |
| first_indexed | 2024-03-11T22:04:58Z |
| format | Article |
| id | doaj.art-8ae369e134044f46a02efa60ce6e2e51 |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-03-11T22:04:58Z |
| publishDate | 2023-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-8ae369e134044f46a02efa60ce6e2e512023-09-25T06:07:50ZengSciendoPolish Journal of Microbiology2544-46462023-09-0172332533710.33073/pjm-2023-034Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root RotLi Si0Zhang Fu-Mei1Shang Xiao-Jing2Hou Rui3College of Forestry, Guizhou University, Guiyang, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaCollege of Forestry, Guizhou University, Guiyang, ChinaFusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot.https://doi.org/10.33073/pjm-2023-034trichoderma asperellumblueberry root rotbiocontrol mechanismspot tests |
| spellingShingle | Li Si Zhang Fu-Mei Shang Xiao-Jing Hou Rui Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot Polish Journal of Microbiology trichoderma asperellum blueberry root rot biocontrol mechanisms pot tests |
| title | Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot |
| title_full | Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot |
| title_fullStr | Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot |
| title_full_unstemmed | Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot |
| title_short | Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot |
| title_sort | control effect and mechanism of trichoderma asperellum tm11 against blueberry root rot |
| topic | trichoderma asperellum blueberry root rot biocontrol mechanisms pot tests |
| url | https://doi.org/10.33073/pjm-2023-034 |
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