Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens
Excessive use of herbicides decreases soil biodiversity and fertility. The literature on the xenobiotic response by microorganisms is focused on herbicide biodegradation as a selective event. Non-degradation systems independent of selection could allow the survival of tolerant bacteria in contaminat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022012269 |
| _version_ | 1811188029700702208 |
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| author | Elizangela Paz de Oliveira Kathleen Evelyn Marchi Janaina Emiliano Stella Marys Christóforo Hinojosa Salazar Alisson Henrique Ferri Rafael Mazer Etto Péricles Martim Reche Sônia Alvim Veiga Pileggi Karlos Henrique Martins Kalks Marcos Rogério Tótola Zelinda Schemczssen-Graeff Marcos Pileggi |
| author_facet | Elizangela Paz de Oliveira Kathleen Evelyn Marchi Janaina Emiliano Stella Marys Christóforo Hinojosa Salazar Alisson Henrique Ferri Rafael Mazer Etto Péricles Martim Reche Sônia Alvim Veiga Pileggi Karlos Henrique Martins Kalks Marcos Rogério Tótola Zelinda Schemczssen-Graeff Marcos Pileggi |
| author_sort | Elizangela Paz de Oliveira |
| collection | DOAJ |
| description | Excessive use of herbicides decreases soil biodiversity and fertility. The literature on the xenobiotic response by microorganisms is focused on herbicide biodegradation as a selective event. Non-degradation systems independent of selection could allow the survival of tolerant bacteria in contaminated environments, impacting xenobiotic turnover and, consequently, bioremediation strategies. However, it is uncertain whether the response based on these systems requires selective pressure to be effective. The objective here was to analyze non-degradation phenotypes, enzymatic and structural response systems, of Pseudomonas fluorescens CMA-55 strain, already investigated the production pattern of quorum sensing molecules in response to glyphosate, not present at the isolation site. One mode of response was associated with decrease in membrane permeability and effective antioxidative response for 0–2.30 mM glyphosate, at the mid-log growing phase, with higher activities of Mn-SOD, KatA, and KatB, and presence of fatty acids as nonadecylic acid, margaric and lauric acid. The second response system was characterized by lower antioxidative enzymes activity, presence of KatC isoform, and pelargonic, capric, myristic, stearic, palmitoleic and palmitic acid as principal fatty acids, allowing the strain to face stressful conditions in 9.20–11.50 mM glyphosate at the stationary phase. Therefore, the bacterial strain could modify the fatty acid composition and the permeability of membranes in two response modes according to the herbicide concentration, even glyphosate was not previously selective for P. fluorescens, featuring a generalist system based on physiological plasticity. |
| first_indexed | 2024-04-11T14:13:39Z |
| format | Article |
| id | doaj.art-8ae4a72584a74cd88876060f5e6f7df9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T14:13:39Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8ae4a72584a74cd88876060f5e6f7df92022-12-22T04:19:37ZengElsevierHeliyon2405-84402022-08-0188e09938Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescensElizangela Paz de Oliveira0Kathleen Evelyn Marchi1Janaina Emiliano2Stella Marys Christóforo Hinojosa Salazar3Alisson Henrique Ferri4Rafael Mazer Etto5Péricles Martim Reche6Sônia Alvim Veiga Pileggi7Karlos Henrique Martins Kalks8Marcos Rogério Tótola9Zelinda Schemczssen-Graeff10Marcos Pileggi11Department of Biotechnology, Genetics and Cell Biology, Maringá State University, Maringá, Paraná, BrazilDepartment of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Microbiology, Londrina State University, Londrina, Paraná, BrazilDepartment of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Chemistry, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Nursing and Public Health, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, BrazilDepartment of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, BrazilDepartment of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, BrazilDepartment of Cellular Biology, Federal University of Paraná, Curitiba, Paraná, BrazilDepartment of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil; Corresponding author.Excessive use of herbicides decreases soil biodiversity and fertility. The literature on the xenobiotic response by microorganisms is focused on herbicide biodegradation as a selective event. Non-degradation systems independent of selection could allow the survival of tolerant bacteria in contaminated environments, impacting xenobiotic turnover and, consequently, bioremediation strategies. However, it is uncertain whether the response based on these systems requires selective pressure to be effective. The objective here was to analyze non-degradation phenotypes, enzymatic and structural response systems, of Pseudomonas fluorescens CMA-55 strain, already investigated the production pattern of quorum sensing molecules in response to glyphosate, not present at the isolation site. One mode of response was associated with decrease in membrane permeability and effective antioxidative response for 0–2.30 mM glyphosate, at the mid-log growing phase, with higher activities of Mn-SOD, KatA, and KatB, and presence of fatty acids as nonadecylic acid, margaric and lauric acid. The second response system was characterized by lower antioxidative enzymes activity, presence of KatC isoform, and pelargonic, capric, myristic, stearic, palmitoleic and palmitic acid as principal fatty acids, allowing the strain to face stressful conditions in 9.20–11.50 mM glyphosate at the stationary phase. Therefore, the bacterial strain could modify the fatty acid composition and the permeability of membranes in two response modes according to the herbicide concentration, even glyphosate was not previously selective for P. fluorescens, featuring a generalist system based on physiological plasticity.http://www.sciencedirect.com/science/article/pii/S2405844022012269HerbicideOxidative enzymePhysiological plasticityOxidative stress responseLipidic peroxidationSelective pressure |
| spellingShingle | Elizangela Paz de Oliveira Kathleen Evelyn Marchi Janaina Emiliano Stella Marys Christóforo Hinojosa Salazar Alisson Henrique Ferri Rafael Mazer Etto Péricles Martim Reche Sônia Alvim Veiga Pileggi Karlos Henrique Martins Kalks Marcos Rogério Tótola Zelinda Schemczssen-Graeff Marcos Pileggi Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens Heliyon Herbicide Oxidative enzyme Physiological plasticity Oxidative stress response Lipidic peroxidation Selective pressure |
| title | Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens |
| title_full | Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens |
| title_fullStr | Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens |
| title_full_unstemmed | Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens |
| title_short | Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens |
| title_sort | changes in fatty acid composition as a response to glyphosate toxicity in pseudomonas fluorescens |
| topic | Herbicide Oxidative enzyme Physiological plasticity Oxidative stress response Lipidic peroxidation Selective pressure |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022012269 |
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