Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions
Abstract Objective Oligodendrocytes (OL) are the glial cell type in the CNS that are responsible for myelin formation. The ability to culture OLs in vitro has provided critical insights into the mechanisms underlying their function. However, primary OL cultures are tedious to obtain, difficult to pr...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-023-06432-w |
| _version_ | 1827635711689359360 |
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| author | Guillermo Rodriguez Bey Quasar Saleem Padiath |
| author_facet | Guillermo Rodriguez Bey Quasar Saleem Padiath |
| author_sort | Guillermo Rodriguez Bey |
| collection | DOAJ |
| description | Abstract Objective Oligodendrocytes (OL) are the glial cell type in the CNS that are responsible for myelin formation. The ability to culture OLs in vitro has provided critical insights into the mechanisms underlying their function. However, primary OL cultures are tedious to obtain, difficult to propagate and are not easily conducive to genetic manipulation. To overcome these obstacles, researchers have generated immortalized OL like cell lines derived from various species. One such cell line is the mouse Oli-neu line which is thought to recapitulate characteristics of OLs in early stages of maturity. They have been extensively utilized in multiple studies as surrogates for OLs, especially in analyzing epigenetic modifications and regulatory pathways in the OL lineage. Results In this report we present the development of optimized culture media and growth conditions that greatly facilitate the differentiation of Oli-neu cells. Oli-neu cells differentiated using these new protocols exhibit a higher expression of myelin related genes and increased branching, both of which are defining characteristics of mature OLs, when compared to previous culture protocols. We envision that these new culture conditions will greatly facilitate the use of Oli-neu cells and enhance their ability to recapitulate the salient features of primary OLs. |
| first_indexed | 2024-03-09T15:31:20Z |
| format | Article |
| id | doaj.art-8ae6a2db559c43d6b0db247352d9ea4a |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-09T15:31:20Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-8ae6a2db559c43d6b0db247352d9ea4a2023-11-26T12:14:51ZengBMCBMC Research Notes1756-05002023-08-011611510.1186/s13104-023-06432-wEnhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditionsGuillermo Rodriguez Bey0Quasar Saleem Padiath1Department of Human Genetics, School of Public Health, University of PittsburghDepartment of Human Genetics, School of Public Health, University of PittsburghAbstract Objective Oligodendrocytes (OL) are the glial cell type in the CNS that are responsible for myelin formation. The ability to culture OLs in vitro has provided critical insights into the mechanisms underlying their function. However, primary OL cultures are tedious to obtain, difficult to propagate and are not easily conducive to genetic manipulation. To overcome these obstacles, researchers have generated immortalized OL like cell lines derived from various species. One such cell line is the mouse Oli-neu line which is thought to recapitulate characteristics of OLs in early stages of maturity. They have been extensively utilized in multiple studies as surrogates for OLs, especially in analyzing epigenetic modifications and regulatory pathways in the OL lineage. Results In this report we present the development of optimized culture media and growth conditions that greatly facilitate the differentiation of Oli-neu cells. Oli-neu cells differentiated using these new protocols exhibit a higher expression of myelin related genes and increased branching, both of which are defining characteristics of mature OLs, when compared to previous culture protocols. We envision that these new culture conditions will greatly facilitate the use of Oli-neu cells and enhance their ability to recapitulate the salient features of primary OLs.https://doi.org/10.1186/s13104-023-06432-wOligodendrocyteCell cultureMyelinOli-neu |
| spellingShingle | Guillermo Rodriguez Bey Quasar Saleem Padiath Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions BMC Research Notes Oligodendrocyte Cell culture Myelin Oli-neu |
| title | Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions |
| title_full | Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions |
| title_fullStr | Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions |
| title_full_unstemmed | Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions |
| title_short | Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions |
| title_sort | enhanced differentiation of the mouse oli neu oligodendroglial cell line using optimized culture conditions |
| topic | Oligodendrocyte Cell culture Myelin Oli-neu |
| url | https://doi.org/10.1186/s13104-023-06432-w |
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