Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets
To evaluate the effects of weaning age on intestinal mucosal morphology, expressions of tight junction proteins and inflammatory cytokines, as well as secretory IgA (sIgA) of Wuzhishan piglets, a total of 96 piglets at 1-day old with an average body weight of 0.56 ± 0.07 kg were randomly divided int...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-10-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2018.1426397 |
| _version_ | 1819108676401627136 |
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| author | Wenjuan Xun Liguang Shi Hanlin Zhou Guanyu Hou Ting Cao |
| author_facet | Wenjuan Xun Liguang Shi Hanlin Zhou Guanyu Hou Ting Cao |
| author_sort | Wenjuan Xun |
| collection | DOAJ |
| description | To evaluate the effects of weaning age on intestinal mucosal morphology, expressions of tight junction proteins and inflammatory cytokines, as well as secretory IgA (sIgA) of Wuzhishan piglets, a total of 96 piglets at 1-day old with an average body weight of 0.56 ± 0.07 kg were randomly divided into four treatments with four replicates per treatment and 6 pigs per replicate. Piglets in those four treatments were weaned at the age of 21(21W), 28(28W), 35 (35W) and 42 (42W) days, respectively. Blood sample was collected for determining plasma diamine oxidase (DAO) and D-lactate at 28, 35, 42, 49 and 56 days old. The jejunum and ileum segments were collected for morphology analysis, and mucosa samples were collected for Real-time PCR and sIgA measurements at 56 days old. The results showed that: compared with the 21W groups, piglets in 35W and 42W groups significantly decreased DAO activity and D-lactate level at 28 to 35 days and 56 days old (p < .05), increased mRNA levels of occludin and zonula occluden-1 (ZO-1) in jejunal and ileal mucosa (p < .05) and claudin in ileal mucosa (p < .05) and reduced the sIgA contents in jejunum and ileum (p < .05) at 56 days old. By contrary, the intestinal mucosal morphology and mRNA levels of cytokines in jejuna and ileal mucosa at 56 days old were not significantly different among the treatments (p > .05). Results suggest that weaning at 35 or 42 days old may be beneficial to intestinal barrier function, whereas weaning at 21 days old promoted sIgA expression. |
| first_indexed | 2024-12-22T03:13:43Z |
| format | Article |
| id | doaj.art-8ae6dcaaaec3425fbcb161a1db0cd34a |
| institution | Directory Open Access Journal |
| issn | 1828-051X |
| language | English |
| last_indexed | 2024-12-22T03:13:43Z |
| publishDate | 2018-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-8ae6dcaaaec3425fbcb161a1db0cd34a2022-12-21T18:40:52ZengTaylor & Francis GroupItalian Journal of Animal Science1828-051X2018-10-0117497698310.1080/1828051X.2018.14263971426397Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini pigletsWenjuan Xun0Liguang Shi1Hanlin Zhou2Guanyu Hou3Ting Cao4Chinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesTo evaluate the effects of weaning age on intestinal mucosal morphology, expressions of tight junction proteins and inflammatory cytokines, as well as secretory IgA (sIgA) of Wuzhishan piglets, a total of 96 piglets at 1-day old with an average body weight of 0.56 ± 0.07 kg were randomly divided into four treatments with four replicates per treatment and 6 pigs per replicate. Piglets in those four treatments were weaned at the age of 21(21W), 28(28W), 35 (35W) and 42 (42W) days, respectively. Blood sample was collected for determining plasma diamine oxidase (DAO) and D-lactate at 28, 35, 42, 49 and 56 days old. The jejunum and ileum segments were collected for morphology analysis, and mucosa samples were collected for Real-time PCR and sIgA measurements at 56 days old. The results showed that: compared with the 21W groups, piglets in 35W and 42W groups significantly decreased DAO activity and D-lactate level at 28 to 35 days and 56 days old (p < .05), increased mRNA levels of occludin and zonula occluden-1 (ZO-1) in jejunal and ileal mucosa (p < .05) and claudin in ileal mucosa (p < .05) and reduced the sIgA contents in jejunum and ileum (p < .05) at 56 days old. By contrary, the intestinal mucosal morphology and mRNA levels of cytokines in jejuna and ileal mucosa at 56 days old were not significantly different among the treatments (p > .05). Results suggest that weaning at 35 or 42 days old may be beneficial to intestinal barrier function, whereas weaning at 21 days old promoted sIgA expression.http://dx.doi.org/10.1080/1828051X.2018.1426397Weaning ageintestinal mucosal morphologytight junction proteinscytokinessecretory IgA |
| spellingShingle | Wenjuan Xun Liguang Shi Hanlin Zhou Guanyu Hou Ting Cao Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets Italian Journal of Animal Science Weaning age intestinal mucosal morphology tight junction proteins cytokines secretory IgA |
| title | Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets |
| title_full | Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets |
| title_fullStr | Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets |
| title_full_unstemmed | Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets |
| title_short | Effect of weaning age on intestinal mucosal morphology, permeability, gene expression of tight junction proteins, cytokines and secretory IgA in Wuzhishan mini piglets |
| title_sort | effect of weaning age on intestinal mucosal morphology permeability gene expression of tight junction proteins cytokines and secretory iga in wuzhishan mini piglets |
| topic | Weaning age intestinal mucosal morphology tight junction proteins cytokines secretory IgA |
| url | http://dx.doi.org/10.1080/1828051X.2018.1426397 |
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