Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase
Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-rene...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/59222 |
| _version_ | 1828375803519303680 |
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| author | Marco Geymonat Qiuran Peng Zhiang Guo Zulin Yu Jay R Unruh Sue L Jaspersen Marisa Segal |
| author_facet | Marco Geymonat Qiuran Peng Zhiang Guo Zulin Yu Jay R Unruh Sue L Jaspersen Marisa Segal |
| author_sort | Marco Geymonat |
| collection | DOAJ |
| description | Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate. |
| first_indexed | 2024-04-14T07:50:57Z |
| format | Article |
| id | doaj.art-8aeab2261ead469682d26e8b35cfa745 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:50:57Z |
| publishDate | 2020-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8aeab2261ead469682d26e8b35cfa7452022-12-22T02:05:11ZengeLife Sciences Publications LtdeLife2050-084X2020-08-01910.7554/eLife.59222Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinaseMarco Geymonat0https://orcid.org/0000-0002-8792-0517Qiuran Peng1https://orcid.org/0000-0002-4761-0944Zhiang Guo2Zulin Yu3Jay R Unruh4https://orcid.org/0000-0003-3077-4990Sue L Jaspersen5https://orcid.org/0000-0001-8312-7063Marisa Segal6https://orcid.org/0000-0003-1848-9388Department of Genetics, University of Cambridge, Cambridge, United KingdomDepartment of Genetics, University of Cambridge, Cambridge, United KingdomDepartment of Genetics, University of Cambridge, Cambridge, United KingdomStowers Institute for Medical Research, Kansas City, United StatesStowers Institute for Medical Research, Kansas City, United StatesStowers Institute for Medical Research, Kansas City, United States; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, United StatesDepartment of Genetics, University of Cambridge, Cambridge, United KingdomAsymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate.https://elifesciences.org/articles/59222spindlepolarityasymmetric fategamma-tubulincell cycle |
| spellingShingle | Marco Geymonat Qiuran Peng Zhiang Guo Zulin Yu Jay R Unruh Sue L Jaspersen Marisa Segal Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase eLife spindle polarity asymmetric fate gamma-tubulin cell cycle |
| title | Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase |
| title_full | Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase |
| title_fullStr | Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase |
| title_full_unstemmed | Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase |
| title_short | Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase |
| title_sort | orderly assembly underpinning built in asymmetry in the yeast centrosome duplication cycle requires cyclin dependent kinase |
| topic | spindle polarity asymmetric fate gamma-tubulin cell cycle |
| url | https://elifesciences.org/articles/59222 |
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