Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders
Plasmodesmata (PD) play a key role in loading of sugars into the phloem. In plant species that employ the so-called active symplasmic loading strategy, sucrose that diffuses into their unique intermediary cells is converted into sugar oligomers. According to the prevalent hypothesis, the oligomers a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00207/full |
| _version_ | 1818559953004134400 |
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| author | Johannes eLiesche Alexander eSchulz |
| author_facet | Johannes eLiesche Alexander eSchulz |
| author_sort | Johannes eLiesche |
| collection | DOAJ |
| description | Plasmodesmata (PD) play a key role in loading of sugars into the phloem. In plant species that employ the so-called active symplasmic loading strategy, sucrose that diffuses into their unique intermediary cells is converted into sugar oligomers. According to the prevalent hypothesis, the oligomers are too large to pass back through PD on the bundle sheath side, but can pass on into the sieve element to be transported in the phloem. Here, we investigate if the PD at the bundle sheath-intermediary cell interface can indeed fulfill the function of blocking transport of sugar oligomers while still enabling efficient diffusion of sucrose. Hindrance factors are derived via theoretical modeling for different PD substructure configurations: sub-nano channels, slit and hydrogel. The results suggest that a strong discrimination could only be realized when the PD opening is almost as small as the sugar oligomers. In order to find model parameters that match the in vivo situation, we measured the effective diffusion coefficient across the interface in question in Cucurbita pepo with 3D-photoactivation microscopy. Calculations indicate that a PD substructure of several sub-nano channels with a radius around 7 Å, a 10.4 Å-wide slit or a hydrogel with 49% polymer fraction would be compatible with the effective diffusion coefficient.If these configurations can accommodate sufficient flux of sucrose into the intermediary cell, while blocking raffinose and stachyose movement was assessed using literature data. While the slit-configuration would efficiently prevent the sugar oligomers from leaking from intermediary cells, none of the configurations could enable a diffusional sucrose flux that matches reported rates at a physiologically relevant concentration potential.The data provides a first insight on how the substructure of PD could enable selective transport, but indicates that additional factors are involved in efficient phloem loading in active symplasmic loading species. |
| first_indexed | 2024-12-14T00:32:13Z |
| format | Article |
| id | doaj.art-8aeaf559cea54a5585855e5fae9e5dbe |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T00:32:13Z |
| publishDate | 2013-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8aeaf559cea54a5585855e5fae9e5dbe2022-12-21T23:24:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-06-01410.3389/fpls.2013.0020751261Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loadersJohannes eLiesche0Alexander eSchulz1University of CopenhagenUniversity of CopenhagenPlasmodesmata (PD) play a key role in loading of sugars into the phloem. In plant species that employ the so-called active symplasmic loading strategy, sucrose that diffuses into their unique intermediary cells is converted into sugar oligomers. According to the prevalent hypothesis, the oligomers are too large to pass back through PD on the bundle sheath side, but can pass on into the sieve element to be transported in the phloem. Here, we investigate if the PD at the bundle sheath-intermediary cell interface can indeed fulfill the function of blocking transport of sugar oligomers while still enabling efficient diffusion of sucrose. Hindrance factors are derived via theoretical modeling for different PD substructure configurations: sub-nano channels, slit and hydrogel. The results suggest that a strong discrimination could only be realized when the PD opening is almost as small as the sugar oligomers. In order to find model parameters that match the in vivo situation, we measured the effective diffusion coefficient across the interface in question in Cucurbita pepo with 3D-photoactivation microscopy. Calculations indicate that a PD substructure of several sub-nano channels with a radius around 7 Å, a 10.4 Å-wide slit or a hydrogel with 49% polymer fraction would be compatible with the effective diffusion coefficient.If these configurations can accommodate sufficient flux of sucrose into the intermediary cell, while blocking raffinose and stachyose movement was assessed using literature data. While the slit-configuration would efficiently prevent the sugar oligomers from leaking from intermediary cells, none of the configurations could enable a diffusional sucrose flux that matches reported rates at a physiologically relevant concentration potential.The data provides a first insight on how the substructure of PD could enable selective transport, but indicates that additional factors are involved in efficient phloem loading in active symplasmic loading species.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00207/fullPlasmodesmataPhloem loadingcarbon allocationPolymer trapHindered diffusion |
| spellingShingle | Johannes eLiesche Alexander eSchulz Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders Frontiers in Plant Science Plasmodesmata Phloem loading carbon allocation Polymer trap Hindered diffusion |
| title | Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| title_full | Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| title_fullStr | Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| title_full_unstemmed | Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| title_short | Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| title_sort | modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders |
| topic | Plasmodesmata Phloem loading carbon allocation Polymer trap Hindered diffusion |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00207/full |
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