Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.)
Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
2017-01-01
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| Series: | Agronomía Colombiana |
| Online Access: | https://revistas.unal.edu.co/index.php/agrocol/article/view/61330 |
| _version_ | 1819063503833530368 |
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| author | Paola Andrea Murillo-Gómez Rodrigo Hoyos S Paul Chavarriaga |
| author_facet | Paola Andrea Murillo-Gómez Rodrigo Hoyos S Paul Chavarriaga |
| author_sort | Paola Andrea Murillo-Gómez |
| collection | DOAJ |
| description | Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic transformation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots. |
| first_indexed | 2024-12-21T15:15:43Z |
| format | Article |
| id | doaj.art-8af4245c455244bd8085b7dbf7ca0bd9 |
| institution | Directory Open Access Journal |
| issn | 0120-9965 2357-3732 |
| language | English |
| last_indexed | 2024-12-21T15:15:43Z |
| publishDate | 2017-01-01 |
| publisher | Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia |
| record_format | Article |
| series | Agronomía Colombiana |
| spelling | doaj.art-8af4245c455244bd8085b7dbf7ca0bd92022-12-21T18:59:10ZengCentro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de ColombiaAgronomía Colombiana0120-99652357-37322017-01-0135151110.15446/agron.colomb.v35n1.6133045772Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.)Paola Andrea Murillo-GómezRodrigo Hoyos SPaul ChavarriagaTree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic transformation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots.https://revistas.unal.edu.co/index.php/agrocol/article/view/61330 |
| spellingShingle | Paola Andrea Murillo-Gómez Rodrigo Hoyos S Paul Chavarriaga Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) Agronomía Colombiana |
| title | Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) |
| title_full | Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) |
| title_fullStr | Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) |
| title_full_unstemmed | Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) |
| title_short | Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.) |
| title_sort | organogenesis in vitro using three tissues types of tree tomato i cyphomandra betacea i cav sendtn |
| url | https://revistas.unal.edu.co/index.php/agrocol/article/view/61330 |
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