Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection
The efficiency of simultaneous treatment of the cold atmospheric pressure plasma jet (CAP) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was investigated. A CAP with a thin and long plume was generated with Ar gas and applied to a common oral bacterium, <i>Enteroco...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/5/3324 |
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| author | Ku Youn Baik Hyun Jo Se Hoon Ki Gi-Chung Kwon Guangsup Cho |
| author_facet | Ku Youn Baik Hyun Jo Se Hoon Ki Gi-Chung Kwon Guangsup Cho |
| author_sort | Ku Youn Baik |
| collection | DOAJ |
| description | The efficiency of simultaneous treatment of the cold atmospheric pressure plasma jet (CAP) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was investigated. A CAP with a thin and long plume was generated with Ar gas and applied to a common oral bacterium, <i>Enterococcus faecalis</i> (<i>E. faecalis</i>). The bactericidal efficiency was evaluated with the electron microscopy and the colony forming unit (CFU) assay. The underlying mechanisms were studied by measuring extracellular chemical changes in the water solution and by measuring biological responses such as the trans-membrane potential, the intracellular oxidative stress, and the membrane permeability. The combination of CAP with H<sub>2</sub>O<sub>2</sub> could provide dramatic synergistic effects in bacterial disinfection through the enhanced membrane transportation of reactive species and the oxidation of intracellular molecules. Since the byproducts of both H<sub>2</sub>O<sub>2</sub> and CAP are not significantly toxic, the synergistic bactericidal effects of their combination could be a good candidate to clinical applications. |
| first_indexed | 2024-03-11T07:29:52Z |
| format | Article |
| id | doaj.art-8af4c59ce00f4af884d16e6fead0d13b |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T07:29:52Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8af4c59ce00f4af884d16e6fead0d13b2023-11-17T07:22:17ZengMDPI AGApplied Sciences2076-34172023-03-01135332410.3390/app13053324Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial DisinfectionKu Youn Baik0Hyun Jo1Se Hoon Ki2Gi-Chung Kwon3Guangsup Cho4Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwanwoon-Ro, Nowon-Gu, Seoul 01897, Republic of KoreaDepartment of Electrical and Biological Physics, Kwangwoon University, 20 Kwanwoon-Ro, Nowon-Gu, Seoul 01897, Republic of KoreaPlasma Bio Team 2, Plasma Bio Research Division, Institute of Plasma Technology, Korea Institute of Fusion Energy, 37 Dongjansan-Ro, Gunsan-si 54004, Republic of KoreaDepartment of Electrical and Biological Physics, Kwangwoon University, 20 Kwanwoon-Ro, Nowon-Gu, Seoul 01897, Republic of KoreaDepartment of Electrical and Biological Physics, Kwangwoon University, 20 Kwanwoon-Ro, Nowon-Gu, Seoul 01897, Republic of KoreaThe efficiency of simultaneous treatment of the cold atmospheric pressure plasma jet (CAP) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was investigated. A CAP with a thin and long plume was generated with Ar gas and applied to a common oral bacterium, <i>Enterococcus faecalis</i> (<i>E. faecalis</i>). The bactericidal efficiency was evaluated with the electron microscopy and the colony forming unit (CFU) assay. The underlying mechanisms were studied by measuring extracellular chemical changes in the water solution and by measuring biological responses such as the trans-membrane potential, the intracellular oxidative stress, and the membrane permeability. The combination of CAP with H<sub>2</sub>O<sub>2</sub> could provide dramatic synergistic effects in bacterial disinfection through the enhanced membrane transportation of reactive species and the oxidation of intracellular molecules. Since the byproducts of both H<sub>2</sub>O<sub>2</sub> and CAP are not significantly toxic, the synergistic bactericidal effects of their combination could be a good candidate to clinical applications.https://www.mdpi.com/2076-3417/13/5/3324cold atmospheric pressure plasma jethydrogen peroxidehurdle principle<i>Enterococcus faecalis</i> |
| spellingShingle | Ku Youn Baik Hyun Jo Se Hoon Ki Gi-Chung Kwon Guangsup Cho Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection Applied Sciences cold atmospheric pressure plasma jet hydrogen peroxide hurdle principle <i>Enterococcus faecalis</i> |
| title | Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection |
| title_full | Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection |
| title_fullStr | Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection |
| title_full_unstemmed | Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection |
| title_short | Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection |
| title_sort | synergistic effect of hydrogen peroxide and cold atmospheric pressure plasma jet for microbial disinfection |
| topic | cold atmospheric pressure plasma jet hydrogen peroxide hurdle principle <i>Enterococcus faecalis</i> |
| url | https://www.mdpi.com/2076-3417/13/5/3324 |
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