Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human
<p>Abstract</p> <p>Background</p> <p>Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-12-01
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| Series: | BMC Genomics |
| _version_ | 1818915442672009216 |
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| author | Pervaiz Shazib Yue Loo Kumar Alan Venkatachalam Gireedhar Clement Marie Sakharkar Meena |
| author_facet | Pervaiz Shazib Yue Loo Kumar Alan Venkatachalam Gireedhar Clement Marie Sakharkar Meena |
| author_sort | Pervaiz Shazib |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro.</p> <p>Results</p> <p>Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR.</p> <p>Conclusion</p> <p>Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding.</p> |
| first_indexed | 2024-12-20T00:02:21Z |
| format | Article |
| id | doaj.art-8af6081ba72a45418468989e1b4c16e1 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-20T00:02:21Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-8af6081ba72a45418468989e1b4c16e12022-12-21T20:00:47ZengBMCBMC Genomics1471-21642009-12-0110Suppl 3S510.1186/1471-2164-10-S3-S5Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of HumanPervaiz ShazibYue LooKumar AlanVenkatachalam GireedharClement MarieSakharkar Meena<p>Abstract</p> <p>Background</p> <p>Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro.</p> <p>Results</p> <p>Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR.</p> <p>Conclusion</p> <p>Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding.</p> |
| spellingShingle | Pervaiz Shazib Yue Loo Kumar Alan Venkatachalam Gireedhar Clement Marie Sakharkar Meena Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human BMC Genomics |
| title | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_full | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_fullStr | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_full_unstemmed | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_short | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_sort | computational identification and experimental validation of ppre motifs in nhe1 and mnsod genes of human |
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