Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress
Although estuarine diatoms have a wide range of salt tolerance, they are often severely stressed by elevated salt concentrations. It remains poorly understood how estuarine diatoms maintain ionic homeostasis under high-salinity conditions. Using a scanning ion-selective electrode technique, this stu...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Changping Chen Xiao Hu Yahui Gao Junrong Liang Lin Sun |
| author_facet | Changping Chen Xiao Hu Yahui Gao Junrong Liang Lin Sun |
| author_sort | Changping Chen |
| collection | DOAJ |
| description | Although estuarine diatoms have a wide range of salt tolerance, they are often severely stressed by elevated salt concentrations. It remains poorly understood how estuarine diatoms maintain ionic homeostasis under high-salinity conditions. Using a scanning ion-selective electrode technique, this study determined the fluxes of H<sup>+</sup>, Na<sup>+</sup>, and K<sup>+</sup> involved in the acclimatization of the estuarine diatom <i>Coscinodiscus centralis</i> Ehrenberg after an elevation in salinity from 15 psu to 35 psu. The <i>C. centralis</i> cells exhibited marked H<sup>+</sup> effluxes after a transient treatment (TT, 30 min) and short-term treatment (ST, 24 h). However, a drastic shift of H<sup>+</sup> efflux toward an influx was induced in the long-term treatment (LT, 10 days). The Na<sup>+</sup> flux under TT, ST, and LT salinity conditions was found to accelerate the Na<sup>+</sup> efflux. More pronounced effects were observed under the ST and LT salinity conditions compared to the TT salinity condition. The K<sup>+</sup> influx showed a significant increase under the LT salinity condition. However, the salinity-induced Na<sup>+</sup>/H<sup>+</sup> exchange in the estuarine diatom was inhibited by amiloride and sodium orthovanadate. These results indicate that the Na<sup>+</sup> extrusion in salt-stressed cells is mainly the result of an active Na<sup>+</sup>/H<sup>+</sup> antiport across the plasma membrane. The pattern of ion fluxes under the TT and ST salinity conditions were different from those under the LT salinity conditions, suggesting an incomplete regulation of the acclimation process in the estuarine diatom under short-term salinity stress. |
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| spelling | doaj.art-8af67bb2619243cfaf1a59711a7eb24f2023-11-19T11:01:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181368310.3390/ijms241813683Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity StressChangping Chen0Xiao Hu1Yahui Gao2Junrong Liang3Lin Sun4Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaKey Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaKey Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaKey Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, ChinaAlthough estuarine diatoms have a wide range of salt tolerance, they are often severely stressed by elevated salt concentrations. It remains poorly understood how estuarine diatoms maintain ionic homeostasis under high-salinity conditions. Using a scanning ion-selective electrode technique, this study determined the fluxes of H<sup>+</sup>, Na<sup>+</sup>, and K<sup>+</sup> involved in the acclimatization of the estuarine diatom <i>Coscinodiscus centralis</i> Ehrenberg after an elevation in salinity from 15 psu to 35 psu. The <i>C. centralis</i> cells exhibited marked H<sup>+</sup> effluxes after a transient treatment (TT, 30 min) and short-term treatment (ST, 24 h). However, a drastic shift of H<sup>+</sup> efflux toward an influx was induced in the long-term treatment (LT, 10 days). The Na<sup>+</sup> flux under TT, ST, and LT salinity conditions was found to accelerate the Na<sup>+</sup> efflux. More pronounced effects were observed under the ST and LT salinity conditions compared to the TT salinity condition. The K<sup>+</sup> influx showed a significant increase under the LT salinity condition. However, the salinity-induced Na<sup>+</sup>/H<sup>+</sup> exchange in the estuarine diatom was inhibited by amiloride and sodium orthovanadate. These results indicate that the Na<sup>+</sup> extrusion in salt-stressed cells is mainly the result of an active Na<sup>+</sup>/H<sup>+</sup> antiport across the plasma membrane. The pattern of ion fluxes under the TT and ST salinity conditions were different from those under the LT salinity conditions, suggesting an incomplete regulation of the acclimation process in the estuarine diatom under short-term salinity stress.https://www.mdpi.com/1422-0067/24/18/13683estuarine diatomsalinityion fluxthe scanning ion-selective electrode technique |
| spellingShingle | Changping Chen Xiao Hu Yahui Gao Junrong Liang Lin Sun Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress International Journal of Molecular Sciences estuarine diatom salinity ion flux the scanning ion-selective electrode technique |
| title | Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress |
| title_full | Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress |
| title_fullStr | Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress |
| title_full_unstemmed | Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress |
| title_short | Ion fluxes Involved in the Adaptation of the Estuarine Diatom <i>Coscinodiscus centralis</i> Ehrenberg to Salinity Stress |
| title_sort | ion fluxes involved in the adaptation of the estuarine diatom i coscinodiscus centralis i ehrenberg to salinity stress |
| topic | estuarine diatom salinity ion flux the scanning ion-selective electrode technique |
| url | https://www.mdpi.com/1422-0067/24/18/13683 |
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