Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir
The rise of coalbed methane bioengineering enables the conversion and utilization of carbon dioxide through microbial action and the carbon cycle. The environment of underground coal reservoirs is the result of a comprehensive effort by microorganisms. Some studies on reservoir microorganisms have p...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1657 |
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| author | Yang Li Jian Chen Shuheng Tang Zhaodong Xi |
| author_facet | Yang Li Jian Chen Shuheng Tang Zhaodong Xi |
| author_sort | Yang Li |
| collection | DOAJ |
| description | The rise of coalbed methane bioengineering enables the conversion and utilization of carbon dioxide through microbial action and the carbon cycle. The environment of underground coal reservoirs is the result of a comprehensive effort by microorganisms. Some studies on reservoir microorganisms have progressed in laboratory conditions. However, it does not replicate the interaction between microorganisms and the environment on site. Hydraulic fracturing is an engineering technology to improve the natural permeability of tight reservoirs and is also a prerequisite for increasing biomethane production. In addition to expanding the pore and fracture systems of coal reservoirs, hydraulic fracturing also improves the living conditions of microbial communities in underground space. The characteristics of microbial communities in the reservoir after hydraulic fracturing are unclear. To this end, we applied the 16S rRNA sequencing technique to coalbed methane production water after hydraulic fracturing south of the Qinshui Basin to analyze the microbial response of the hydraulic fracturing process in the coal reservoir. The diversity of microbial communities associated with organic degradation was improved after hydraulic fracturing in the coal reservoir. The proportion of Actinobacteria in the reservoir water of the study area increased significantly, and the abundance of Aminicenantes and Planctomycetes increased, which do not exist in non-fracturing coalbed methane wells or exist at very low abundance. There are different types of methanogens in the study area, especially in fracturing wells. Ecological factors also determine the metabolic pathway of methanogens in coal seams. After hydraulic fracturing, the impact on the reservoir’s microbial communities remains within months. Hydraulic fracturing can strengthen the carbon circulation process, thereby enhancing the block’s methane and carbon dioxide circulation. The study provides a unique theoretical basis for microbially enhanced coalbed methane. |
| first_indexed | 2024-03-11T00:48:59Z |
| format | Article |
| id | doaj.art-8af6b165d20d419d8c97caa908428f01 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:48:59Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8af6b165d20d419d8c97caa908428f012023-11-18T20:34:36ZengMDPI AGMicroorganisms2076-26072023-06-01117165710.3390/microorganisms11071657Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal ReservoirYang Li0Jian Chen1Shuheng Tang2Zhaodong Xi3School of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Energy Resource, China University of Geosciences, Beijing 100083, ChinaSchool of Energy Resource, China University of Geosciences, Beijing 100083, ChinaThe rise of coalbed methane bioengineering enables the conversion and utilization of carbon dioxide through microbial action and the carbon cycle. The environment of underground coal reservoirs is the result of a comprehensive effort by microorganisms. Some studies on reservoir microorganisms have progressed in laboratory conditions. However, it does not replicate the interaction between microorganisms and the environment on site. Hydraulic fracturing is an engineering technology to improve the natural permeability of tight reservoirs and is also a prerequisite for increasing biomethane production. In addition to expanding the pore and fracture systems of coal reservoirs, hydraulic fracturing also improves the living conditions of microbial communities in underground space. The characteristics of microbial communities in the reservoir after hydraulic fracturing are unclear. To this end, we applied the 16S rRNA sequencing technique to coalbed methane production water after hydraulic fracturing south of the Qinshui Basin to analyze the microbial response of the hydraulic fracturing process in the coal reservoir. The diversity of microbial communities associated with organic degradation was improved after hydraulic fracturing in the coal reservoir. The proportion of Actinobacteria in the reservoir water of the study area increased significantly, and the abundance of Aminicenantes and Planctomycetes increased, which do not exist in non-fracturing coalbed methane wells or exist at very low abundance. There are different types of methanogens in the study area, especially in fracturing wells. Ecological factors also determine the metabolic pathway of methanogens in coal seams. After hydraulic fracturing, the impact on the reservoir’s microbial communities remains within months. Hydraulic fracturing can strengthen the carbon circulation process, thereby enhancing the block’s methane and carbon dioxide circulation. The study provides a unique theoretical basis for microbially enhanced coalbed methane.https://www.mdpi.com/2076-2607/11/7/1657coalbed methanebiogeochemistrymethanogensmicrobial diversitythe Qinshui Basin |
| spellingShingle | Yang Li Jian Chen Shuheng Tang Zhaodong Xi Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir Microorganisms coalbed methane biogeochemistry methanogens microbial diversity the Qinshui Basin |
| title | Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir |
| title_full | Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir |
| title_fullStr | Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir |
| title_full_unstemmed | Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir |
| title_short | Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir |
| title_sort | microbial communities affected by hydraulic fracturing and environmental factors within an in situ coal reservoir |
| topic | coalbed methane biogeochemistry methanogens microbial diversity the Qinshui Basin |
| url | https://www.mdpi.com/2076-2607/11/7/1657 |
| work_keys_str_mv | AT yangli microbialcommunitiesaffectedbyhydraulicfracturingandenvironmentalfactorswithinaninsitucoalreservoir AT jianchen microbialcommunitiesaffectedbyhydraulicfracturingandenvironmentalfactorswithinaninsitucoalreservoir AT shuhengtang microbialcommunitiesaffectedbyhydraulicfracturingandenvironmentalfactorswithinaninsitucoalreservoir AT zhaodongxi microbialcommunitiesaffectedbyhydraulicfracturingandenvironmentalfactorswithinaninsitucoalreservoir |