An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes
<p>Abstract</p> <p>Background</p> <p>Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. The present study was performed to apply a serum antibody microarray to screen for differentially regulated c...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/10/1/71 |
| _version_ | 1818229963240767488 |
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| author | Mahlknecht Philipp Stemberger Sylvia Sprenger Fabienne Rainer Johannes Hametner Eva Kirchmair Rudolf Grabmer Christoph Scherfler Christoph Wenning Gregor K Seppi Klaus Poewe Werner Reindl Markus |
| author_facet | Mahlknecht Philipp Stemberger Sylvia Sprenger Fabienne Rainer Johannes Hametner Eva Kirchmair Rudolf Grabmer Christoph Scherfler Christoph Wenning Gregor K Seppi Klaus Poewe Werner Reindl Markus |
| author_sort | Mahlknecht Philipp |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson's disease (PD), multiple system atrophy (MSA), progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS).</p> <p>Results</p> <p>Serum samples were obtained from patients with clinical diagnoses of PD (n = 117), MSA (n = 31) and PSP/CBS (n = 38) and 99 controls. Cytokine profiles of sera from patients and controls were analyzed with a semiquantitative human antibody array for 174 cytokines and the expression of 12 cytokines was found to be significantly altered. In a next step, results from the microarray experiment were individually validated by different immunoassays. Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD. However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.</p> <p>Conclusions</p> <p>In our unbiased cytokine array based screening approach and validation by a different immunoassay only two of 174 cytokines were significantly altered between patients and controls.</p> |
| first_indexed | 2024-12-12T10:26:57Z |
| format | Article |
| id | doaj.art-8af6e1c66c1a45c8bff64bc49c086db7 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-12T10:26:57Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-8af6e1c66c1a45c8bff64bc49c086db72022-12-22T00:27:26ZengBMCProteome Science1477-59562012-11-011017110.1186/1477-5956-10-71An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromesMahlknecht PhilippStemberger SylviaSprenger FabienneRainer JohannesHametner EvaKirchmair RudolfGrabmer ChristophScherfler ChristophWenning Gregor KSeppi KlausPoewe WernerReindl Markus<p>Abstract</p> <p>Background</p> <p>Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson's disease (PD), multiple system atrophy (MSA), progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS).</p> <p>Results</p> <p>Serum samples were obtained from patients with clinical diagnoses of PD (n = 117), MSA (n = 31) and PSP/CBS (n = 38) and 99 controls. Cytokine profiles of sera from patients and controls were analyzed with a semiquantitative human antibody array for 174 cytokines and the expression of 12 cytokines was found to be significantly altered. In a next step, results from the microarray experiment were individually validated by different immunoassays. Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD. However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.</p> <p>Conclusions</p> <p>In our unbiased cytokine array based screening approach and validation by a different immunoassay only two of 174 cytokines were significantly altered between patients and controls.</p>http://www.proteomesci.com/content/10/1/71 |
| spellingShingle | Mahlknecht Philipp Stemberger Sylvia Sprenger Fabienne Rainer Johannes Hametner Eva Kirchmair Rudolf Grabmer Christoph Scherfler Christoph Wenning Gregor K Seppi Klaus Poewe Werner Reindl Markus An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes Proteome Science |
| title | An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes |
| title_full | An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes |
| title_fullStr | An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes |
| title_full_unstemmed | An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes |
| title_short | An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes |
| title_sort | antibody microarray analysis of serum cytokines in neurodegenerative parkinsonian syndromes |
| url | http://www.proteomesci.com/content/10/1/71 |
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