Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains
Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. Howev...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-11-01
|
| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545823400023 |
| _version_ | 1797449773359300608 |
|---|---|
| author | Ke Wang Wanjian Zhang Xiangquan Deng Shen Tong Hui Cheng Mengyuan Qin Lei Zheng Kun Zhao Ruizhan Zhai Zhongqing Jia Ping Qiu |
| author_facet | Ke Wang Wanjian Zhang Xiangquan Deng Shen Tong Hui Cheng Mengyuan Qin Lei Zheng Kun Zhao Ruizhan Zhai Zhongqing Jia Ping Qiu |
| author_sort | Ke Wang |
| collection | DOAJ |
| description | Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo, we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828[Formula: see text]nm and 620[Formula: see text]nm, respectively. Both experimental and simulation results show that as the imaging depth increases, NIR emission decays less than orange-red fluorescent emission. These results show that it is preferable to shift the emission wavelength to NIR to enable more efficient signal collection deep in the brain. |
| first_indexed | 2024-03-09T14:29:55Z |
| format | Article |
| id | doaj.art-8afa03895e8e44968cf125d36ea9970c |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-09T14:29:55Z |
| publishDate | 2023-11-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-8afa03895e8e44968cf125d36ea9970c2023-11-28T03:24:33ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-11-01160610.1142/S1793545823400023Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brainsKe Wang0Wanjian Zhang1Xiangquan Deng2Shen Tong3Hui Cheng4Mengyuan Qin5Lei Zheng6Kun Zhao7Ruizhan Zhai8Zhongqing Jia9Ping Qiu10Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaLaser Institute, Qilu University of Technology (Shandong Academy of Sciences), Qingdao Shandong 266000, P. R. ChinaLaser Institute, Qilu University of Technology (Shandong Academy of Sciences), Qingdao Shandong 266000, P. R. ChinaLaser Institute, Qilu University of Technology (Shandong Academy of Sciences), Qingdao Shandong 266000, P. R. ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. ChinaMultiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo, we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828[Formula: see text]nm and 620[Formula: see text]nm, respectively. Both experimental and simulation results show that as the imaging depth increases, NIR emission decays less than orange-red fluorescent emission. These results show that it is preferable to shift the emission wavelength to NIR to enable more efficient signal collection deep in the brain.https://www.worldscientific.com/doi/10.1142/S17935458234000233-Photon microscopyemission fluorescenceFM4-64Monte Carlo |
| spellingShingle | Ke Wang Wanjian Zhang Xiangquan Deng Shen Tong Hui Cheng Mengyuan Qin Lei Zheng Kun Zhao Ruizhan Zhai Zhongqing Jia Ping Qiu Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains Journal of Innovative Optical Health Sciences 3-Photon microscopy emission fluorescence FM4-64 Monte Carlo |
| title | Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains |
| title_full | Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains |
| title_fullStr | Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains |
| title_full_unstemmed | Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains |
| title_short | Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains |
| title_sort | comparison of the emission wavelengths by a single fluorescent dye on in vivo 3 photon imaging of mouse brains |
| topic | 3-Photon microscopy emission fluorescence FM4-64 Monte Carlo |
| url | https://www.worldscientific.com/doi/10.1142/S1793545823400023 |
| work_keys_str_mv | AT kewang comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT wanjianzhang comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT xiangquandeng comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT shentong comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT huicheng comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT mengyuanqin comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT leizheng comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT kunzhao comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT ruizhanzhai comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT zhongqingjia comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains AT pingqiu comparisonoftheemissionwavelengthsbyasinglefluorescentdyeoninvivo3photonimagingofmousebrains |