The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique
A rumen simulation technique apparatus with eight 800 mL fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treatments cons...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-03-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/3121 |
| _version_ | 1811214210543124480 |
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| author | Xiang H. Zhao Jian M. Gong Shan Zhou Chan J. Liu Ming R. Qu |
| author_facet | Xiang H. Zhao Jian M. Gong Shan Zhou Chan J. Liu Ming R. Qu |
| author_sort | Xiang H. Zhao |
| collection | DOAJ |
| description | A rumen simulation technique apparatus with eight 800 mL fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treatments consisted of two NFC types (starch and inulin) supplemented with 0 g/d (low RDP) or 1.56 g/d (high RDP) sodium caseinate. No significant differences existed among dietary treatments in the apparent disappearance of dietary nutrients except for dietary N, which increased with increased dietary RDP (P<0.001). Compared with starch, inulin treatments reduced the molar proportion of acetate (P<0.001), the acetate:propionate ratio (P<0.001), and methane production (P=0.006), but increased the butyrate proportion (P<0.001). Increased dietary RDP led to increases in production of total volatile fatty acid (P=0.014) and methane (P=0.050), various measures of N (P≤0.046), and 16s rDNA copy numbers of <em>Ruminococcus flavefaciens</em> (P≤0.010). Non-fibre carbohydrate source did not affect daily microbial N flow regardless of dietary RDP, but ammonia N production was lower for inulin than for starch treatments under high RDP conditions (P<0.001). Compared with starch treatments, inulin depressed the copy numbers of <em>Fibrobacter succinogenes</em> in solid fraction (P=0.023) and <em>R. flavefaciens</em> in liquid (P=0.017) and solid fractions (P=0.007), but it increased the carboxymethylcellulase activity in solid fraction (P=0.045). Current results suggest that starch and inulin differ in ruminal volatile fatty acid fermentation but have similar effects on ruminal digestion and microbial synthesis <em>in vitro</em>, although inulin suppressed the growth of partial ruminal cellulolytic bacteria. |
| first_indexed | 2024-04-12T05:59:26Z |
| format | Article |
| id | doaj.art-8afa73e648fb496e983a926925a45387 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-04-12T05:59:26Z |
| publishDate | 2014-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-8afa73e648fb496e983a926925a453872022-12-22T03:45:05ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2014-03-0113110.4081/ijas.2014.31212216The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation techniqueXiang H. Zhao0Jian M. Gong1Shan Zhou2Chan J. Liu3Ming R. Qu4College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang CityCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang CityCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang CityCollege of Animal Science and Technology, Northwest A&F University, XianyangCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang CityA rumen simulation technique apparatus with eight 800 mL fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treatments consisted of two NFC types (starch and inulin) supplemented with 0 g/d (low RDP) or 1.56 g/d (high RDP) sodium caseinate. No significant differences existed among dietary treatments in the apparent disappearance of dietary nutrients except for dietary N, which increased with increased dietary RDP (P<0.001). Compared with starch, inulin treatments reduced the molar proportion of acetate (P<0.001), the acetate:propionate ratio (P<0.001), and methane production (P=0.006), but increased the butyrate proportion (P<0.001). Increased dietary RDP led to increases in production of total volatile fatty acid (P=0.014) and methane (P=0.050), various measures of N (P≤0.046), and 16s rDNA copy numbers of <em>Ruminococcus flavefaciens</em> (P≤0.010). Non-fibre carbohydrate source did not affect daily microbial N flow regardless of dietary RDP, but ammonia N production was lower for inulin than for starch treatments under high RDP conditions (P<0.001). Compared with starch treatments, inulin depressed the copy numbers of <em>Fibrobacter succinogenes</em> in solid fraction (P=0.023) and <em>R. flavefaciens</em> in liquid (P=0.017) and solid fractions (P=0.007), but it increased the carboxymethylcellulase activity in solid fraction (P=0.045). Current results suggest that starch and inulin differ in ruminal volatile fatty acid fermentation but have similar effects on ruminal digestion and microbial synthesis <em>in vitro</em>, although inulin suppressed the growth of partial ruminal cellulolytic bacteria.http://www.aspajournal.it/index.php/ijas/article/view/3121Microbial growth, Non-fibre carbohydrate, Rumen degradable protein, Ruminal fermentation, Rusitec |
| spellingShingle | Xiang H. Zhao Jian M. Gong Shan Zhou Chan J. Liu Ming R. Qu The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique Italian Journal of Animal Science Microbial growth, Non-fibre carbohydrate, Rumen degradable protein, Ruminal fermentation, Rusitec |
| title | The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| title_full | The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| title_fullStr | The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| title_full_unstemmed | The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| title_short | The effect of starch, inulin, and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| title_sort | effect of starch inulin and degradable protein on ruminal fermentation and microbial growth in rumen simulation technique |
| topic | Microbial growth, Non-fibre carbohydrate, Rumen degradable protein, Ruminal fermentation, Rusitec |
| url | http://www.aspajournal.it/index.php/ijas/article/view/3121 |
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