Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation
The sugar composition and content of fruit have a significant impact on their flavor and taste. In pitaya, or dragon fruit, sweetness is a crucial determinant of fruit taste and consumer preference. The sugars will eventually be exported transporters (SWEETs), a novel group of sugar transporters tha...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12882 |
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| author | Rui Jiang Liangfang Wu Jianmei Zeng Kamran Shah Rong Zhang Guibing Hu Yonghua Qin Zhike Zhang |
| author_facet | Rui Jiang Liangfang Wu Jianmei Zeng Kamran Shah Rong Zhang Guibing Hu Yonghua Qin Zhike Zhang |
| author_sort | Rui Jiang |
| collection | DOAJ |
| description | The sugar composition and content of fruit have a significant impact on their flavor and taste. In pitaya, or dragon fruit, sweetness is a crucial determinant of fruit taste and consumer preference. The sugars will eventually be exported transporters (SWEETs), a novel group of sugar transporters that have various physiological functions, including phloem loading, seed filling, nectar secretion, and fruit development. However, the role of SWEETs in sugar accumulation in pitaya fruit is not yet clear. Here, we identified 19 potential members (<i>HuSWEET</i> genes) of the <i>SWEET</i> family in pitaya and analyzed their conserved motifs, physiochemical characteristics, chromosomal distribution, gene structure, and phylogenetic relationship. Seven highly conserved α-helical transmembrane domains (7-TMs) were found, and the HuSWEET proteins can be divided into three clades based on the phylogenetic analysis. Interestingly, we found two <i>HuSWEET</i> genes, <i>HuSWEET12a</i> and <i>HuSWEET13d</i>, that showed strong preferential expressions in fruits and an upward trend during fruit maturation, suggesting they have key roles in sugar accumulation in pitaya. This can be further roughly demonstrated by the fact that transgenic tomato plants overexpressing <i>HuSWEET12a</i>/<i>13d</i> accumulated high levels of sugar in the mature fruit. Together, our result provides new insights into the regulation of sugar accumulation by <i>SWEET</i> family genes in pitaya fruit, which also set a crucial basis for the further functional study of the <i>HuSWEETs.</i> |
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| language | English |
| last_indexed | 2024-03-10T23:52:29Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8afc0ffc25ea45dc8992d4375dadec832023-11-19T01:31:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161288210.3390/ijms241612882Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar AccumulationRui Jiang0Liangfang Wu1Jianmei Zeng2Kamran Shah3Rong Zhang4Guibing Hu5Yonghua Qin6Zhike Zhang7Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaThe sugar composition and content of fruit have a significant impact on their flavor and taste. In pitaya, or dragon fruit, sweetness is a crucial determinant of fruit taste and consumer preference. The sugars will eventually be exported transporters (SWEETs), a novel group of sugar transporters that have various physiological functions, including phloem loading, seed filling, nectar secretion, and fruit development. However, the role of SWEETs in sugar accumulation in pitaya fruit is not yet clear. Here, we identified 19 potential members (<i>HuSWEET</i> genes) of the <i>SWEET</i> family in pitaya and analyzed their conserved motifs, physiochemical characteristics, chromosomal distribution, gene structure, and phylogenetic relationship. Seven highly conserved α-helical transmembrane domains (7-TMs) were found, and the HuSWEET proteins can be divided into three clades based on the phylogenetic analysis. Interestingly, we found two <i>HuSWEET</i> genes, <i>HuSWEET12a</i> and <i>HuSWEET13d</i>, that showed strong preferential expressions in fruits and an upward trend during fruit maturation, suggesting they have key roles in sugar accumulation in pitaya. This can be further roughly demonstrated by the fact that transgenic tomato plants overexpressing <i>HuSWEET12a</i>/<i>13d</i> accumulated high levels of sugar in the mature fruit. Together, our result provides new insights into the regulation of sugar accumulation by <i>SWEET</i> family genes in pitaya fruit, which also set a crucial basis for the further functional study of the <i>HuSWEETs.</i>https://www.mdpi.com/1422-0067/24/16/12882pitayagenome-wide<i>SWEET</i> genes familyfruit |
| spellingShingle | Rui Jiang Liangfang Wu Jianmei Zeng Kamran Shah Rong Zhang Guibing Hu Yonghua Qin Zhike Zhang Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation International Journal of Molecular Sciences pitaya genome-wide <i>SWEET</i> genes family fruit |
| title | Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation |
| title_full | Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation |
| title_fullStr | Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation |
| title_full_unstemmed | Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation |
| title_short | Identification of <i>HuSWEET</i> Family in Pitaya (<i>Hylocereus undatus</i>) and Key Roles of <i>HuSWEET12a</i> and <i>HuSWEET13d</i> in Sugar Accumulation |
| title_sort | identification of i husweet i family in pitaya i hylocereus undatus i and key roles of i husweet12a i and i husweet13d i in sugar accumulation |
| topic | pitaya genome-wide <i>SWEET</i> genes family fruit |
| url | https://www.mdpi.com/1422-0067/24/16/12882 |
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