Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer
Abstract Lactococcus lactis, a lactic acid bacterium with a typical fermentative metabolism, can also use oxygen as an extracellular electron acceptor. Here we demonstrate, for the first time, that L. lactis blocked in NAD+ regeneration can use the alternative electron acceptor ferricyanide to suppo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14229 |
| _version_ | 1797818578488000512 |
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| author | Liuyan Gu Xinxin Xiao Ge Zhao Paul Kempen Shuangqing Zhao Jianming Liu Sang Yup Lee Christian Solem |
| author_facet | Liuyan Gu Xinxin Xiao Ge Zhao Paul Kempen Shuangqing Zhao Jianming Liu Sang Yup Lee Christian Solem |
| author_sort | Liuyan Gu |
| collection | DOAJ |
| description | Abstract Lactococcus lactis, a lactic acid bacterium with a typical fermentative metabolism, can also use oxygen as an extracellular electron acceptor. Here we demonstrate, for the first time, that L. lactis blocked in NAD+ regeneration can use the alternative electron acceptor ferricyanide to support growth. By electrochemical analysis and characterization of strains carrying mutations in the respiratory chain, we pinpoint the essential role of the NADH dehydrogenase and 2‐amino‐3‐carboxy‐1,4‐naphtoquinone in extracellular electron transfer (EET) and uncover the underlying pathway systematically. Ferricyanide respiration has unexpected effects on L. lactis, e.g., we find that morphology is altered from the normal coccoid to a more rod shaped appearance, and that acid resistance is increased. Using adaptive laboratory evolution (ALE), we successfully enhance the capacity for EET. Whole‐genome sequencing reveals the underlying reason for the observed enhanced EET capacity to be a late‐stage blocking of menaquinone biosynthesis. The perspectives of the study are numerous, especially within food fermentation and microbiome engineering, where EET can help relieve oxidative stress, promote growth of oxygen sensitive microorganisms and play critical roles in shaping microbial communities. |
| first_indexed | 2024-03-13T09:09:59Z |
| format | Article |
| id | doaj.art-8afd3d0400904821bdbbe366b904eb74 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-13T09:09:59Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-8afd3d0400904821bdbbe366b904eb742023-05-27T09:33:44ZengWileyMicrobial Biotechnology1751-79152023-06-011661277129210.1111/1751-7915.14229Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transferLiuyan Gu0Xinxin Xiao1Ge Zhao2Paul Kempen3Shuangqing Zhao4Jianming Liu5Sang Yup Lee6Christian Solem7National Food Institute Technical University of Denmark Kongens Lyngby DenmarkDepartment of Chemistry and Bioscience Aalborg University Aalborg DenmarkNational Food Institute Technical University of Denmark Kongens Lyngby DenmarkDepartment of Health Technology Technical University of Denmark Kongens Lyngby DenmarkNational Food Institute Technical University of Denmark Kongens Lyngby DenmarkNational Food Institute Technical University of Denmark Kongens Lyngby DenmarkDepartment of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of KoreaNational Food Institute Technical University of Denmark Kongens Lyngby DenmarkAbstract Lactococcus lactis, a lactic acid bacterium with a typical fermentative metabolism, can also use oxygen as an extracellular electron acceptor. Here we demonstrate, for the first time, that L. lactis blocked in NAD+ regeneration can use the alternative electron acceptor ferricyanide to support growth. By electrochemical analysis and characterization of strains carrying mutations in the respiratory chain, we pinpoint the essential role of the NADH dehydrogenase and 2‐amino‐3‐carboxy‐1,4‐naphtoquinone in extracellular electron transfer (EET) and uncover the underlying pathway systematically. Ferricyanide respiration has unexpected effects on L. lactis, e.g., we find that morphology is altered from the normal coccoid to a more rod shaped appearance, and that acid resistance is increased. Using adaptive laboratory evolution (ALE), we successfully enhance the capacity for EET. Whole‐genome sequencing reveals the underlying reason for the observed enhanced EET capacity to be a late‐stage blocking of menaquinone biosynthesis. The perspectives of the study are numerous, especially within food fermentation and microbiome engineering, where EET can help relieve oxidative stress, promote growth of oxygen sensitive microorganisms and play critical roles in shaping microbial communities.https://doi.org/10.1111/1751-7915.14229 |
| spellingShingle | Liuyan Gu Xinxin Xiao Ge Zhao Paul Kempen Shuangqing Zhao Jianming Liu Sang Yup Lee Christian Solem Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer Microbial Biotechnology |
| title | Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer |
| title_full | Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer |
| title_fullStr | Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer |
| title_full_unstemmed | Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer |
| title_short | Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer |
| title_sort | rewiring the respiratory pathway of lactococcus lactis to enhance extracellular electron transfer |
| url | https://doi.org/10.1111/1751-7915.14229 |
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