Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato

Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato

Plant gene targeting (GT) can be utilized to precisely replace up to several kilobases of a plant genome. Recent studies using the powerful clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nucleases significantly improved plant GT efficiency. However, GT...

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Bibliographic Details
Main Authors: Tien Van Vu, Duong Thi Hai Doan, Mil Thi Tran, Yeon Woo Sung, Young Jong Song, Jae-Yean Kim
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Plant Science
Subjects:
LbCas12a
gene targeting
gene editing
homology-directed repair
SpCas9
geminiviral replicon
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.722552/full

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