Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i>
Intracellular polyols are used as osmoprotectants by many plants under environmental stress. However, few studies have shown the role of polyol transporters in the tolerance of plants to abiotic stresses. Here, we describe the expression characteristics and potential functions of <i>Lotus japo...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5149 |
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| author | Jiao Liu Leru Liu Lu Tian Shaoming Xu Guojiang Wu Huawu Jiang Yaping Chen |
| author_facet | Jiao Liu Leru Liu Lu Tian Shaoming Xu Guojiang Wu Huawu Jiang Yaping Chen |
| author_sort | Jiao Liu |
| collection | DOAJ |
| description | Intracellular polyols are used as osmoprotectants by many plants under environmental stress. However, few studies have shown the role of polyol transporters in the tolerance of plants to abiotic stresses. Here, we describe the expression characteristics and potential functions of <i>Lotus japonicus</i> polyol transporter LjPLT3 under salt stress. Using <i>LjPLT3</i> promoter-reporter gene plants showed that <i>LjPLT3</i> was expressed in the vascular tissue of <i>L. japonicus</i> leaf, stem, root, and nodule. The expression was also induced by NaCl treatment. Overexpression of <i>LjPLT3</i> in <i>L. japonicus</i> modified the growth rate and saline tolerance of the transgenic plants. The <i>OELjPLT3</i> seedlings displayed reduced plant height under both nitrogen-sufficient and symbiotic nitrogen fixation conditions when 4 weeks old. The nodule number of <i>OELjPLT3</i> plants was reduced by 6.7–27.4% when 4 weeks old. After exposure to a NaCl treatment in Petri dishes for 10 days, <i>OELjPLT3</i> seedlings had a higher chlorophyll concentration, fresh weight, and survival rate than those in the wild type. For symbiotic nitrogen fixation conditions, the decrease in nitrogenase activity of <i>OELjPLT3</i> plants was slower than that of the wild type after salt treatment. Compared to the wild type, both the accumulation of small organic molecules and the activity of antioxidant enzymes were higher under salt stress. Considering the concentration of lower reactive oxygen species (ROS) in transgenic lines, we speculate that overexpression of <i>LjPLT3</i> in <i>L. japonicus</i> might improve the ROS scavenging system to alleviate the oxidative damage caused by salt stress, thereby increasing plant salinity tolerance. Our results will direct the breeding of forage legumes in saline land and also provide an opportunity for the improvement of poor and saline soils. |
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| spelling | doaj.art-8b032ce362fb4b858565cff6bfcf3c002023-11-17T11:29:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246514910.3390/ijms24065149Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i>Jiao Liu0Leru Liu1Lu Tian2Shaoming Xu3Guojiang Wu4Huawu Jiang5Yaping Chen6Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaIntracellular polyols are used as osmoprotectants by many plants under environmental stress. However, few studies have shown the role of polyol transporters in the tolerance of plants to abiotic stresses. Here, we describe the expression characteristics and potential functions of <i>Lotus japonicus</i> polyol transporter LjPLT3 under salt stress. Using <i>LjPLT3</i> promoter-reporter gene plants showed that <i>LjPLT3</i> was expressed in the vascular tissue of <i>L. japonicus</i> leaf, stem, root, and nodule. The expression was also induced by NaCl treatment. Overexpression of <i>LjPLT3</i> in <i>L. japonicus</i> modified the growth rate and saline tolerance of the transgenic plants. The <i>OELjPLT3</i> seedlings displayed reduced plant height under both nitrogen-sufficient and symbiotic nitrogen fixation conditions when 4 weeks old. The nodule number of <i>OELjPLT3</i> plants was reduced by 6.7–27.4% when 4 weeks old. After exposure to a NaCl treatment in Petri dishes for 10 days, <i>OELjPLT3</i> seedlings had a higher chlorophyll concentration, fresh weight, and survival rate than those in the wild type. For symbiotic nitrogen fixation conditions, the decrease in nitrogenase activity of <i>OELjPLT3</i> plants was slower than that of the wild type after salt treatment. Compared to the wild type, both the accumulation of small organic molecules and the activity of antioxidant enzymes were higher under salt stress. Considering the concentration of lower reactive oxygen species (ROS) in transgenic lines, we speculate that overexpression of <i>LjPLT3</i> in <i>L. japonicus</i> might improve the ROS scavenging system to alleviate the oxidative damage caused by salt stress, thereby increasing plant salinity tolerance. Our results will direct the breeding of forage legumes in saline land and also provide an opportunity for the improvement of poor and saline soils.https://www.mdpi.com/1422-0067/24/6/5149<i>Lotus japonicus</i>polyol/monosaccharide transporter LjPLT3salinity stressnitrogenase activity |
| spellingShingle | Jiao Liu Leru Liu Lu Tian Shaoming Xu Guojiang Wu Huawu Jiang Yaping Chen Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> International Journal of Molecular Sciences <i>Lotus japonicus</i> polyol/monosaccharide transporter LjPLT3 salinity stress nitrogenase activity |
| title | Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> |
| title_full | Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> |
| title_fullStr | Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> |
| title_full_unstemmed | Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> |
| title_short | Overexpression of <i>LjPLT3</i> Enhances Salt Tolerance in <i>Lotus japonicus</i> |
| title_sort | overexpression of i ljplt3 i enhances salt tolerance in i lotus japonicus i |
| topic | <i>Lotus japonicus</i> polyol/monosaccharide transporter LjPLT3 salinity stress nitrogenase activity |
| url | https://www.mdpi.com/1422-0067/24/6/5149 |
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