Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells
Considering the presence of β-sitosterol in different functional foods and the potential role of neural stem cells (NSCs) in treating neurodegenerative diseases, this study was designed to evaluate the effects of oral administration of β-sitosterol on proliferation and differentiation of NSCs. Adult...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614001200 |
| _version_ | 1818871359493636096 |
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| author | Azadeh Hamedi Amir Ghanbari Vahid Saeidi Razieh Razavipour Hassan Azari |
| author_facet | Azadeh Hamedi Amir Ghanbari Vahid Saeidi Razieh Razavipour Hassan Azari |
| author_sort | Azadeh Hamedi |
| collection | DOAJ |
| description | Considering the presence of β-sitosterol in different functional foods and the potential role of neural stem cells (NSCs) in treating neurodegenerative diseases, this study was designed to evaluate the effects of oral administration of β-sitosterol on proliferation and differentiation of NSCs. Adult mice were divided into three groups. For 2 weeks, the control group received vehicle and the low and high dose groups received 33 and 99 mg/kg/day of β-sitosterol, respectively. Harvested NSCs from the subventricular zone (SVZ) were plated in neurosphere culture and the size and number of resulting neurospheres, and also their in vitro differentiation, were evaluated. β-Sitosterol at 99 mg/kg/day significantly increased proliferation of NSCs as evidenced by their neurosphere forming frequency (719.2 ± 53.65) compared to the control group (457.6 ± 21.35). Mean neurosphere diameter was significantly larger in both high and low dose β-sitosterol groups compared to the control group. β-Sitosterol did not affect in vitro differentiation of NSCs. |
| first_indexed | 2024-12-19T12:21:40Z |
| format | Article |
| id | doaj.art-8b0a46e7021f461c8bb5e5020cb307e6 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-19T12:21:40Z |
| publishDate | 2014-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-8b0a46e7021f461c8bb5e5020cb307e62022-12-21T20:21:45ZengElsevierJournal of Functional Foods1756-46462014-05-018252258Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cellsAzadeh Hamedi0Amir Ghanbari1Vahid Saeidi2Razieh Razavipour3Hassan Azari4Medicinal Plants Processing Research Center, Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IranNeural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, Shiraz School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Center Committee, International Branch, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Center Committee, International Branch, Shiraz University of Medical Sciences, Shiraz, IranNeural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, Shiraz School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Neural Stem Cell and Regenerative Neuroscience Laboratory, Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author. Tel.: +98 7112304372.Considering the presence of β-sitosterol in different functional foods and the potential role of neural stem cells (NSCs) in treating neurodegenerative diseases, this study was designed to evaluate the effects of oral administration of β-sitosterol on proliferation and differentiation of NSCs. Adult mice were divided into three groups. For 2 weeks, the control group received vehicle and the low and high dose groups received 33 and 99 mg/kg/day of β-sitosterol, respectively. Harvested NSCs from the subventricular zone (SVZ) were plated in neurosphere culture and the size and number of resulting neurospheres, and also their in vitro differentiation, were evaluated. β-Sitosterol at 99 mg/kg/day significantly increased proliferation of NSCs as evidenced by their neurosphere forming frequency (719.2 ± 53.65) compared to the control group (457.6 ± 21.35). Mean neurosphere diameter was significantly larger in both high and low dose β-sitosterol groups compared to the control group. β-Sitosterol did not affect in vitro differentiation of NSCs.http://www.sciencedirect.com/science/article/pii/S1756464614001200β-SitosterolNeural stem cellSubventricular zoneProliferationNeurosphere assay |
| spellingShingle | Azadeh Hamedi Amir Ghanbari Vahid Saeidi Razieh Razavipour Hassan Azari Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells Journal of Functional Foods β-Sitosterol Neural stem cell Subventricular zone Proliferation Neurosphere assay |
| title | Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| title_full | Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| title_fullStr | Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| title_full_unstemmed | Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| title_short | Effects of β-sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| title_sort | effects of β sitosterol oral administration on the proliferation and differentiation of neural stem cells |
| topic | β-Sitosterol Neural stem cell Subventricular zone Proliferation Neurosphere assay |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614001200 |
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