Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease
To demonstrate and analyze the specific T-cell response following barrier disruption and antigen translocation, circulating food antigen-specific effector T-cells isolated from peripheral blood were analyzed in patients suffering from celiac disease (CeD) as well as inflammatory bowel disease (IBD)....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8153 |
| _version_ | 1797602452653998080 |
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| author | Yasmina Rodríguez-Sillke Michael Schumann Donata Lissner Federica Branchi Fabian Proft Ulrich Steinhoff Britta Siegmund Rainer Glauben |
| author_facet | Yasmina Rodríguez-Sillke Michael Schumann Donata Lissner Federica Branchi Fabian Proft Ulrich Steinhoff Britta Siegmund Rainer Glauben |
| author_sort | Yasmina Rodríguez-Sillke |
| collection | DOAJ |
| description | To demonstrate and analyze the specific T-cell response following barrier disruption and antigen translocation, circulating food antigen-specific effector T-cells isolated from peripheral blood were analyzed in patients suffering from celiac disease (CeD) as well as inflammatory bowel disease (IBD). We applied the antigen-reactive T-cell enrichment (ARTE) technique allowing for phenotypical and functional flow cytometric analyses of rare nutritional antigen-specific T-cells, including the celiac disease-causing gliadin (gluten). For CeD, patient groups, including treatment-refractory cases, differ significantly from healthy controls. Even symptom-free patients on a gluten-free diet were distinguishable from healthy controls, without being previously challenged with gluten. Moreover, frequency and phenotype of nutritional antigen-specific T-cells of IBD patients directly correlated to the presence of small intestinal inflammation. Specifically, the frequency of antigen specific T-cells as well as pro-inflammatory cytokines was increased in patients with active CeD or Crohn’s disease, respectively. These results suggest active small intestinal inflammation as key for the development of a peripheral food antigen-specific T-cell response in Crohn’s disease and celiac disease. |
| first_indexed | 2024-03-11T04:16:18Z |
| format | Article |
| id | doaj.art-8b0bc96e234d4e8bbfe1149be72853d9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:18Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8b0bc96e234d4e8bbfe1149be72853d92023-11-17T23:05:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249815310.3390/ijms24098153Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel DiseaseYasmina Rodríguez-Sillke0Michael Schumann1Donata Lissner2Federica Branchi3Fabian Proft4Ulrich Steinhoff5Britta Siegmund6Rainer Glauben7Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyInstitute for Medical Microbiology and Hospital Hygiene, Philipps University of Marburg, 35043 Marburg, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyDepartment of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charité-University Medicine Berlin, 13125 Berlin, GermanyTo demonstrate and analyze the specific T-cell response following barrier disruption and antigen translocation, circulating food antigen-specific effector T-cells isolated from peripheral blood were analyzed in patients suffering from celiac disease (CeD) as well as inflammatory bowel disease (IBD). We applied the antigen-reactive T-cell enrichment (ARTE) technique allowing for phenotypical and functional flow cytometric analyses of rare nutritional antigen-specific T-cells, including the celiac disease-causing gliadin (gluten). For CeD, patient groups, including treatment-refractory cases, differ significantly from healthy controls. Even symptom-free patients on a gluten-free diet were distinguishable from healthy controls, without being previously challenged with gluten. Moreover, frequency and phenotype of nutritional antigen-specific T-cells of IBD patients directly correlated to the presence of small intestinal inflammation. Specifically, the frequency of antigen specific T-cells as well as pro-inflammatory cytokines was increased in patients with active CeD or Crohn’s disease, respectively. These results suggest active small intestinal inflammation as key for the development of a peripheral food antigen-specific T-cell response in Crohn’s disease and celiac disease.https://www.mdpi.com/1422-0067/24/9/8153antigen-specific T-cellsceliac diseasegliadinIBDfood antigens |
| spellingShingle | Yasmina Rodríguez-Sillke Michael Schumann Donata Lissner Federica Branchi Fabian Proft Ulrich Steinhoff Britta Siegmund Rainer Glauben Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease International Journal of Molecular Sciences antigen-specific T-cells celiac disease gliadin IBD food antigens |
| title | Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease |
| title_full | Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease |
| title_fullStr | Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease |
| title_full_unstemmed | Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease |
| title_short | Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease |
| title_sort | analysis of circulating food antigen specific t cells in celiac disease and inflammatory bowel disease |
| topic | antigen-specific T-cells celiac disease gliadin IBD food antigens |
| url | https://www.mdpi.com/1422-0067/24/9/8153 |
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