Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean
This study investigated the effects of plasma duration and different reactive species ratios of cold atmospheric-pressure plasma treatment on both physical and endogenous hormone changes in enhancing the germination and growth of mung bean seeds. Seed germination and sprout stem length were signific...
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MDPI AG
2022-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/20/10308 |
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| author | Thi Quynh Xuan Le Linh Nhat Nguyen Thanh Tung Nguyen Eun Ha Choi Quang Liem Nguyen Nagendra Kumar Kaushik Nguyen Thuan Dao |
| author_facet | Thi Quynh Xuan Le Linh Nhat Nguyen Thanh Tung Nguyen Eun Ha Choi Quang Liem Nguyen Nagendra Kumar Kaushik Nguyen Thuan Dao |
| author_sort | Thi Quynh Xuan Le |
| collection | DOAJ |
| description | This study investigated the effects of plasma duration and different reactive species ratios of cold atmospheric-pressure plasma treatment on both physical and endogenous hormone changes in enhancing the germination and growth of mung bean seeds. Seed germination and sprout stem length were significantly enhanced after plasma treatment. The germination rate increased eleven times after 12 h, while the radicles’ length increased ~3 times after 96 h with optimal plasma treatment parameters. SEM images showed that the plasmas directly induced gradual changes in the seed coating, including deformed and shrunken epidermis, and cracks with sizes varying from 0.2 to 1.5 µm after 4 min of plasma treatment. Water contact angle was reduced from 73° with untreated seed to almost 0° with 4 min treated seed. These effects could lead to better water absorption on the surface of treated seeds. We found that a plasma energy dosage of 0.08 Wh per seed and NO concentration between 20–95 ppm were the optimal enhancement conditions. We also showed that, for the first time, through delicate extraction, separation, and quantification processes, NO-induced upregulation of the natural growth hormone gibberellic acid could be the dominant phytochemistry pathway responsible for the enhancement effect. |
| first_indexed | 2024-03-09T20:47:46Z |
| format | Article |
| id | doaj.art-8b0e2d81e2fd481992151854956813ef |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:47:46Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8b0e2d81e2fd481992151854956813ef2023-11-23T22:42:43ZengMDPI AGApplied Sciences2076-34172022-10-0112201030810.3390/app122010308Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung BeanThi Quynh Xuan Le0Linh Nhat Nguyen1Thanh Tung Nguyen2Eun Ha Choi3Quang Liem Nguyen4Nagendra Kumar Kaushik5Nguyen Thuan Dao6Laboratory of Plasma Technology, Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi 100000, VietnamLaboratory of Plasma Technology, Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi 100000, VietnamLaboratory of Plasma Technology, Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi 100000, VietnamPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaLaboratory of Plasma Technology, Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi 100000, VietnamPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaLaboratory of Plasma Technology, Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi 100000, VietnamThis study investigated the effects of plasma duration and different reactive species ratios of cold atmospheric-pressure plasma treatment on both physical and endogenous hormone changes in enhancing the germination and growth of mung bean seeds. Seed germination and sprout stem length were significantly enhanced after plasma treatment. The germination rate increased eleven times after 12 h, while the radicles’ length increased ~3 times after 96 h with optimal plasma treatment parameters. SEM images showed that the plasmas directly induced gradual changes in the seed coating, including deformed and shrunken epidermis, and cracks with sizes varying from 0.2 to 1.5 µm after 4 min of plasma treatment. Water contact angle was reduced from 73° with untreated seed to almost 0° with 4 min treated seed. These effects could lead to better water absorption on the surface of treated seeds. We found that a plasma energy dosage of 0.08 Wh per seed and NO concentration between 20–95 ppm were the optimal enhancement conditions. We also showed that, for the first time, through delicate extraction, separation, and quantification processes, NO-induced upregulation of the natural growth hormone gibberellic acid could be the dominant phytochemistry pathway responsible for the enhancement effect.https://www.mdpi.com/2076-3417/12/20/10308cold plasmaplasma in agricultureseed germination enhancementgibberellic acidSEMLC-MS |
| spellingShingle | Thi Quynh Xuan Le Linh Nhat Nguyen Thanh Tung Nguyen Eun Ha Choi Quang Liem Nguyen Nagendra Kumar Kaushik Nguyen Thuan Dao Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean Applied Sciences cold plasma plasma in agriculture seed germination enhancement gibberellic acid SEM LC-MS |
| title | Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean |
| title_full | Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean |
| title_fullStr | Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean |
| title_full_unstemmed | Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean |
| title_short | Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean |
| title_sort | effects of cold plasma treatment on physical modification and endogenous hormone regulation in enhancing seed germination and radicle growth of mung bean |
| topic | cold plasma plasma in agriculture seed germination enhancement gibberellic acid SEM LC-MS |
| url | https://www.mdpi.com/2076-3417/12/20/10308 |
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