Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates
The emergence of pathogenic bacteria with β-lactam antibiotics-resistant profile has threatened the continued use of such antibiotics in the future. This research was conducted to investigate the antimicrobial activity of green tea ethanol extract (GTE) and its ability to improve the antibacterial a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Ahmad Dahlan
2021-03-01
|
| Series: | Pharmaciana |
| Subjects: | |
| Online Access: | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/16874/pdf_186 |
| _version_ | 1818882934364438528 |
|---|---|
| author | Rezky Yanuarti Firzan Nainu Sartini Sartini |
| author_facet | Rezky Yanuarti Firzan Nainu Sartini Sartini |
| author_sort | Rezky Yanuarti |
| collection | DOAJ |
| description | The emergence of pathogenic bacteria with β-lactam antibiotics-resistant profile has threatened the continued use of such antibiotics in the future. This research was conducted to investigate the antimicrobial activity of green tea ethanol extract (GTE) and its ability to improve the antibacterial action of several β-lactam antibiotics against Enterobacter cloacae clinical isolates. The simplicia of green tea was extracted by sonication for 30 minutes using 50% ethanol solvent, and the total phenolic content of the GTE was subsequently determined. Next, the GTE used in testing against two clinical isolates of E. cloacae was obtained from the Pathology Laboratory of Wahidin Sudiro Husodo Hospital in Makassar. The sensitivity of bacteria to GTE was confirmed using the agar diffusion method, the Vitek® rapid method, and the double-disk synergistic test. Antibacterial activity of antibiotics, GTE, and combination of antibiotics with GTE were then tested against clinical isolates of E. cloacae using the checkerboard microdilution assay. The results showed that GTE contained 51.64 ± 0.21 % measured as gallic acid equivalent and 37.95 + 5.17 % Epigallocatechin gallate (EGCG). The confirmatory test results indicated that one clinical isolate of E. cloacae (code 13/04) was resistant to amoxicillin-clavulanate but did not produce an extended-spectrum β-lactamase (ESBL). Another clinical E. cloacae isolate (code 275B/06) was indicated to produce ESBL and demonstrated to yield resistance to amoxicillin-clavulanate and cefotaxime. The minimum inhibitory concentration of GTE against the two clinical isolates of E. cloacae was >8000 ppm (8 mg/ml). In conclusion, GTE could not increase the antibacterial activity of amoxicillin and cefotaxime, but it was sufficient to improve the activity of imipenem against the tested isolates of E. cloacae. |
| first_indexed | 2024-12-19T15:25:39Z |
| format | Article |
| id | doaj.art-8b0e6b9daab14d32863c62d612516e9d |
| institution | Directory Open Access Journal |
| issn | 2088-4559 2477-0256 |
| language | English |
| last_indexed | 2024-12-19T15:25:39Z |
| publishDate | 2021-03-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj.art-8b0e6b9daab14d32863c62d612516e9d2022-12-21T20:15:54ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562021-03-0111113314210.12928/pharmaciana.v11i1.16874Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolatesRezky Yanuarti0 Firzan Nainu1Sartini Sartini2Magister Program Faculty of Pharmacy, Hasanuddin University; Sekolah Tinggi Ilmu Farmasi PaluFaculty of Pharmacy, Hasanuddin University Faculty of Pharmacy, Hasanuddin University The emergence of pathogenic bacteria with β-lactam antibiotics-resistant profile has threatened the continued use of such antibiotics in the future. This research was conducted to investigate the antimicrobial activity of green tea ethanol extract (GTE) and its ability to improve the antibacterial action of several β-lactam antibiotics against Enterobacter cloacae clinical isolates. The simplicia of green tea was extracted by sonication for 30 minutes using 50% ethanol solvent, and the total phenolic content of the GTE was subsequently determined. Next, the GTE used in testing against two clinical isolates of E. cloacae was obtained from the Pathology Laboratory of Wahidin Sudiro Husodo Hospital in Makassar. The sensitivity of bacteria to GTE was confirmed using the agar diffusion method, the Vitek® rapid method, and the double-disk synergistic test. Antibacterial activity of antibiotics, GTE, and combination of antibiotics with GTE were then tested against clinical isolates of E. cloacae using the checkerboard microdilution assay. The results showed that GTE contained 51.64 ± 0.21 % measured as gallic acid equivalent and 37.95 + 5.17 % Epigallocatechin gallate (EGCG). The confirmatory test results indicated that one clinical isolate of E. cloacae (code 13/04) was resistant to amoxicillin-clavulanate but did not produce an extended-spectrum β-lactamase (ESBL). Another clinical E. cloacae isolate (code 275B/06) was indicated to produce ESBL and demonstrated to yield resistance to amoxicillin-clavulanate and cefotaxime. The minimum inhibitory concentration of GTE against the two clinical isolates of E. cloacae was >8000 ppm (8 mg/ml). In conclusion, GTE could not increase the antibacterial activity of amoxicillin and cefotaxime, but it was sufficient to improve the activity of imipenem against the tested isolates of E. cloacae.http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/16874/pdf_186β-lactam antibioticscamellia sinensis l.antibacterial modulatione. cloacae |
| spellingShingle | Rezky Yanuarti Firzan Nainu Sartini Sartini Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates Pharmaciana β-lactam antibiotics camellia sinensis l. antibacterial modulation e. cloacae |
| title | Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates |
| title_full | Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates |
| title_fullStr | Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates |
| title_full_unstemmed | Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates |
| title_short | Green tea extract-mediated augmentation of imipenem antibacterial activity against Enterobacter cloacae clinical isolates |
| title_sort | green tea extract mediated augmentation of imipenem antibacterial activity against enterobacter cloacae clinical isolates |
| topic | β-lactam antibiotics camellia sinensis l. antibacterial modulation e. cloacae |
| url | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/16874/pdf_186 |
| work_keys_str_mv | AT rezkyyanuarti greenteaextractmediatedaugmentationofimipenemantibacterialactivityagainstenterobactercloacaeclinicalisolates AT firzannainu greenteaextractmediatedaugmentationofimipenemantibacterialactivityagainstenterobactercloacaeclinicalisolates AT sartinisartini greenteaextractmediatedaugmentationofimipenemantibacterialactivityagainstenterobactercloacaeclinicalisolates |