Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens
Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this novel host. In this study, we...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.826142/full |
| _version_ | 1818333601399308288 |
|---|---|
| author | Lisa Tietze Antonia Mangold Maria W. Hoff Rahmi Lale |
| author_facet | Lisa Tietze Antonia Mangold Maria W. Hoff Rahmi Lale |
| author_sort | Lisa Tietze |
| collection | DOAJ |
| description | Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this novel host. In this study, we report the identification and cross-characterisation of artificial promoters and 5′ untranslated regions (artificial regulatory sequence, ARES) that lead to production of fluorescent proteins with a wide-range of expression levels. We identify and cross-characterise 52 constructs in V. natriegens and E. coli. Furthermore, we report the DNA sequence and motif analysis of the ARESs using various algorithms. With this study, we expand the pool of characterised genetic DNA parts that can be used for different biotechnological applications using V. natriegens as a host microorganism. |
| first_indexed | 2024-12-13T13:54:14Z |
| format | Article |
| id | doaj.art-8b0f9bf79653482b888b57ec727a5e89 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T13:54:14Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8b0f9bf79653482b888b57ec727a5e892022-12-21T23:42:58ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-01-011010.3389/fbioe.2022.826142826142Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegensLisa TietzeAntonia MangoldMaria W. HoffRahmi LaleVibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this novel host. In this study, we report the identification and cross-characterisation of artificial promoters and 5′ untranslated regions (artificial regulatory sequence, ARES) that lead to production of fluorescent proteins with a wide-range of expression levels. We identify and cross-characterise 52 constructs in V. natriegens and E. coli. Furthermore, we report the DNA sequence and motif analysis of the ARESs using various algorithms. With this study, we expand the pool of characterised genetic DNA parts that can be used for different biotechnological applications using V. natriegens as a host microorganism.https://www.frontiersin.org/articles/10.3389/fbioe.2022.826142/fullVibrio natriegensEschecrichia coliartificial promoterstranscriptiontranslation5ʹ untranslated region |
| spellingShingle | Lisa Tietze Antonia Mangold Maria W. Hoff Rahmi Lale Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens Frontiers in Bioengineering and Biotechnology Vibrio natriegens Eschecrichia coli artificial promoters transcription translation 5ʹ untranslated region |
| title | Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens |
| title_full | Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens |
| title_fullStr | Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens |
| title_full_unstemmed | Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens |
| title_short | Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens |
| title_sort | identification and cross characterisation of artificial promoters and 5 untranslated regions in vibrio natriegens |
| topic | Vibrio natriegens Eschecrichia coli artificial promoters transcription translation 5ʹ untranslated region |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.826142/full |
| work_keys_str_mv | AT lisatietze identificationandcrosscharacterisationofartificialpromotersand5untranslatedregionsinvibrionatriegens AT antoniamangold identificationandcrosscharacterisationofartificialpromotersand5untranslatedregionsinvibrionatriegens AT mariawhoff identificationandcrosscharacterisationofartificialpromotersand5untranslatedregionsinvibrionatriegens AT rahmilale identificationandcrosscharacterisationofartificialpromotersand5untranslatedregionsinvibrionatriegens |