A Hypothetical Approach to Concentrate Microorganisms from Human Urine Samples Using Paper-Based Adsorbents for Point-of-Care Molecular Assays

A Hypothetical Approach to Concentrate Microorganisms from Human Urine Samples Using Paper-Based Adsorbents for Point-of-Care Molecular Assays

This hypothesis demonstrates that the efficiency of loop-mediated isothermal amplification (LAMP) for nucleic acid detection can be positively influenced by the preconcentration of microbial cells onto hydrophobic paper surfaces. The mechanism of this model is based on the high affinity of microbes...

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Bibliographic Details
Main Authors: Isha Uttam, Sujesh Sudarsan, Rohitraj Ray, Raja Chinnappan, Ahmed Yaqinuddin, Khaled Al-Kattan, Naresh Kumar Mani
Format: Article
Language:English
Published: MDPI AG 2023-12-01
Series:Life
Subjects:
preconcentration
loop-mediated isothermal amplification (LAMP)
urine
microbial adhesion
hydrophobic
paper-based analytical devices (PADs)
Online Access:https://www.mdpi.com/2075-1729/14/1/38

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