2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo
2′-O-galloylhyperin, a major compound of Pyrola incarnata Fisch., possesses a variety of biological and pharmacological activities, including anti-oxidative and anti-inflammatory activities. Nevertheless, the underlying molecular mechanisms of 2′-O-GH in microbial infection and sepsis are not clear....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00679/full |
| _version_ | 1818499821374275584 |
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| author | Peng Wang Chang Gao Na Guo Sun-Dong Zhang Wei Wang Li-Ping Yao Jing Zhang Thomas Efferth Yu-Jie Fu Yu-Jie Fu |
| author_facet | Peng Wang Chang Gao Na Guo Sun-Dong Zhang Wei Wang Li-Ping Yao Jing Zhang Thomas Efferth Yu-Jie Fu Yu-Jie Fu |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | 2′-O-galloylhyperin, a major compound of Pyrola incarnata Fisch., possesses a variety of biological and pharmacological activities, including anti-oxidative and anti-inflammatory activities. Nevertheless, the underlying molecular mechanisms of 2′-O-GH in microbial infection and sepsis are not clear. In this study, we investigated the anti-inflammatory effects of 2′-O-GH. We found that 2′-O-GH significantly reduced the production of TNF-α, IL-6, and nitric oxide (NO), suppressed the expression levels of iNOS, blocked the translocation of NF-κB from the cytosol to nucleus, and decreased the MAPK activation in LPS-activated RAW 264.7 cells. 2′-O-GH also enhanced the nuclear translocation of Nrf2 and up-regulated the expression of heme oxygenase-1 (HO-1) and SIRT1. In addition, the administration of 2′-O-GH attenuated the TNF-α and IL-6 production in the serum, infiltration of inflammatory cells, liver tissue damage, and the mortality rate of LPS-challenged mice. Moreover, 2′-O-GH significantly upregulated Nrf2 and SIRT1 expression and inhibited the inflammatory responses in the liver of septic mice. The collective data indicate that 2′-O-GH could potentially be a novel functional food candidate in the treatment of sepsis. |
| first_indexed | 2024-12-10T20:34:47Z |
| format | Article |
| id | doaj.art-8b12aa5f6576483795a91519cb85d97a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T20:34:47Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8b12aa5f6576483795a91519cb85d97a2022-12-22T01:34:34ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-06-01910.3389/fphar.2018.006793680492′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and VivoPeng Wang0Chang Gao1Na Guo2Sun-Dong Zhang3Wei Wang4Li-Ping Yao5Jing Zhang6Thomas Efferth7Yu-Jie Fu8Yu-Jie Fu9Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaDepartment of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, ChinaKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaInstitute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, GermanyKey Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China2′-O-galloylhyperin, a major compound of Pyrola incarnata Fisch., possesses a variety of biological and pharmacological activities, including anti-oxidative and anti-inflammatory activities. Nevertheless, the underlying molecular mechanisms of 2′-O-GH in microbial infection and sepsis are not clear. In this study, we investigated the anti-inflammatory effects of 2′-O-GH. We found that 2′-O-GH significantly reduced the production of TNF-α, IL-6, and nitric oxide (NO), suppressed the expression levels of iNOS, blocked the translocation of NF-κB from the cytosol to nucleus, and decreased the MAPK activation in LPS-activated RAW 264.7 cells. 2′-O-GH also enhanced the nuclear translocation of Nrf2 and up-regulated the expression of heme oxygenase-1 (HO-1) and SIRT1. In addition, the administration of 2′-O-GH attenuated the TNF-α and IL-6 production in the serum, infiltration of inflammatory cells, liver tissue damage, and the mortality rate of LPS-challenged mice. Moreover, 2′-O-GH significantly upregulated Nrf2 and SIRT1 expression and inhibited the inflammatory responses in the liver of septic mice. The collective data indicate that 2′-O-GH could potentially be a novel functional food candidate in the treatment of sepsis.https://www.frontiersin.org/article/10.3389/fphar.2018.00679/fullNF-κBSIRT1anti-inflammation2′-O-galloylhyperinNrf2 |
| spellingShingle | Peng Wang Chang Gao Na Guo Sun-Dong Zhang Wei Wang Li-Ping Yao Jing Zhang Thomas Efferth Yu-Jie Fu Yu-Jie Fu 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo Frontiers in Pharmacology NF-κB SIRT1 anti-inflammation 2′-O-galloylhyperin Nrf2 |
| title | 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo |
| title_full | 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo |
| title_fullStr | 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo |
| title_full_unstemmed | 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo |
| title_short | 2′-O-Galloylhyperin Isolated From Pyrola incarnata Fisch. Attenuates LPS-Induced Inflammatory Response by Activation of SIRT1/Nrf2 and Inhibition of the NF-κB Pathways in Vitro and Vivo |
| title_sort | 2 o galloylhyperin isolated from pyrola incarnata fisch attenuates lps induced inflammatory response by activation of sirt1 nrf2 and inhibition of the nf κb pathways in vitro and vivo |
| topic | NF-κB SIRT1 anti-inflammation 2′-O-galloylhyperin Nrf2 |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00679/full |
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