Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production
Abstract An anaerobic granular sludge was enriched to utilize H2/CO2 in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation wi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-02-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202200027 |
| _version_ | 1797934834274795520 |
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| author | Yaxue He Chiara Cassarini Piet N.L. Lens |
| author_facet | Yaxue He Chiara Cassarini Piet N.L. Lens |
| author_sort | Yaxue He |
| collection | DOAJ |
| description | Abstract An anaerobic granular sludge was enriched to utilize H2/CO2 in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H2/CO2 was replaced by 100% H2 to induce solventogenesis, whereas butyric acid was produced with the highest concentration of 2.5 mM. At stage III with 10 µM tungsten (W) addition, iso‐valeric acid, valeric acid, and caproic acid were produced at pH 4.5–5.0. In the batch tests inoculated with the enriched sludge taken from the bioreactor (day 70), however, methane production occurred at pH 6. Exogenous 15 mM acetate addition enhanced both the H2 and CO2 consumption rate compared to exogenous 10, 30, and 45 mM acetate by the enriched sludge. Exogenous acetate was failed to be converted to ethanol using H2 as electron donor by the enriched acetogens. |
| first_indexed | 2024-04-10T18:05:05Z |
| format | Article |
| id | doaj.art-8b131dccde8344f7905d08c2dc2afe1d |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-10T18:05:05Z |
| publishDate | 2023-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-8b131dccde8344f7905d08c2dc2afe1d2023-02-02T13:23:41ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632023-02-01232n/an/a10.1002/elsc.202200027Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane productionYaxue He0Chiara Cassarini1Piet N.L. Lens2National University of Ireland Galway Galway IrelandNational University of Ireland Galway Galway IrelandNational University of Ireland Galway Galway IrelandAbstract An anaerobic granular sludge was enriched to utilize H2/CO2 in a continuous gas‐fed up‐flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H2/CO2 was replaced by 100% H2 to induce solventogenesis, whereas butyric acid was produced with the highest concentration of 2.5 mM. At stage III with 10 µM tungsten (W) addition, iso‐valeric acid, valeric acid, and caproic acid were produced at pH 4.5–5.0. In the batch tests inoculated with the enriched sludge taken from the bioreactor (day 70), however, methane production occurred at pH 6. Exogenous 15 mM acetate addition enhanced both the H2 and CO2 consumption rate compared to exogenous 10, 30, and 45 mM acetate by the enriched sludge. Exogenous acetate was failed to be converted to ethanol using H2 as electron donor by the enriched acetogens.https://doi.org/10.1002/elsc.202200027acetogenesisanaerobic sludgeH2/CO2 fermentationmethanogenesissolventogenesis |
| spellingShingle | Yaxue He Chiara Cassarini Piet N.L. Lens Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production Engineering in Life Sciences acetogenesis anaerobic sludge H2/CO2 fermentation methanogenesis solventogenesis |
| title | Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production |
| title_full | Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production |
| title_fullStr | Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production |
| title_full_unstemmed | Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production |
| title_short | Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production |
| title_sort | enrichment of homoacetogens converting h2 co2 into acids and ethanol and simultaneous methane production |
| topic | acetogenesis anaerobic sludge H2/CO2 fermentation methanogenesis solventogenesis |
| url | https://doi.org/10.1002/elsc.202200027 |
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