Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp.
We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.465 |
| _version_ | 1818915179694391296 |
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| author | Colwyn M. Thomas Saijun Tang Kim Hammond-Kosack Jonathan D. G. Jones |
| author_facet | Colwyn M. Thomas Saijun Tang Kim Hammond-Kosack Jonathan D. G. Jones |
| author_sort | Colwyn M. Thomas |
| collection | DOAJ |
| description | We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco. |
| first_indexed | 2024-12-19T23:58:10Z |
| format | Article |
| id | doaj.art-8b151193e5f04fd2958fa2beabc58062 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:10Z |
| publishDate | 2000-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-8b151193e5f04fd2958fa2beabc580622022-12-21T20:00:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-04-0113446546910.1094/MPMI.2000.13.4.465Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp.Colwyn M. ThomasSaijun TangKim Hammond-KosackJonathan D. G. JonesWe have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.465Cf genesdisease resistance |
| spellingShingle | Colwyn M. Thomas Saijun Tang Kim Hammond-Kosack Jonathan D. G. Jones Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. Molecular Plant-Microbe Interactions Cf genes disease resistance |
| title | Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. |
| title_full | Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. |
| title_fullStr | Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. |
| title_full_unstemmed | Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. |
| title_short | Comparison of the Hypersensitive Response Induced by the Tomato Cf-4 and Cf-9 Genes in Nicotiana spp. |
| title_sort | comparison of the hypersensitive response induced by the tomato cf 4 and cf 9 genes in nicotiana spp |
| topic | Cf genes disease resistance |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.465 |
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