Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i>
<i>Lolium multiflorum</i> is widely planted in temperate and subtropical regions globally, and it has high economic value owing to its use as forage grass for a wide variety of livestock and poultry. However, drought seriously restricts its yield and quality. At present, owing to the lac...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/14/7921 |
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| author | Qiuxu Liu Fangyan Wang Yang Shuai Linkai Huang Xinquan Zhang |
| author_facet | Qiuxu Liu Fangyan Wang Yang Shuai Linkai Huang Xinquan Zhang |
| author_sort | Qiuxu Liu |
| collection | DOAJ |
| description | <i>Lolium multiflorum</i> is widely planted in temperate and subtropical regions globally, and it has high economic value owing to its use as forage grass for a wide variety of livestock and poultry. However, drought seriously restricts its yield and quality. At present, owing to the lack of available genomic resources, many types of basic research cannot be conducted, which severely limits the in-depth functional analysis of genes in <i>L. multiflorum</i>. Therefore, we used single-molecule real-time (SMRT) and next-generation sequencing (NGS) to sequence the complex transcriptome of <i>L. multiflorum</i> under drought. We identified 41,141 DEGs in leaves, 35,559 DEGs in roots, respectively. Moreover, we identified 1243 alternative splicing events under drought. LmPIP5K9 produced two different transcripts with opposite expression patterns, possibly through the phospholipid signaling pathway or the negatively regulated sugar-mediated root growth response to drought stress, respectively. Additionally, 13,079 transcription factors in 90 families were obtained. An in-depth analysis of R2R3-MYB gene family members was performed to preliminarily demonstrate their functions by utilizing subcellular localization and overexpression in yeast. Our data make a significant contribution to the genetics of <i>L. multiflorum</i>, offering a current understanding of plant adaptation to drought stress. |
| first_indexed | 2024-03-09T10:17:28Z |
| format | Article |
| id | doaj.art-8b1bd1afe8df4667838dcd1505c821e0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:17:28Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8b1bd1afe8df4667838dcd1505c821e02023-12-01T22:16:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012314792110.3390/ijms23147921Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i>Qiuxu Liu0Fangyan Wang1Yang Shuai2Linkai Huang3Xinquan Zhang4College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China<i>Lolium multiflorum</i> is widely planted in temperate and subtropical regions globally, and it has high economic value owing to its use as forage grass for a wide variety of livestock and poultry. However, drought seriously restricts its yield and quality. At present, owing to the lack of available genomic resources, many types of basic research cannot be conducted, which severely limits the in-depth functional analysis of genes in <i>L. multiflorum</i>. Therefore, we used single-molecule real-time (SMRT) and next-generation sequencing (NGS) to sequence the complex transcriptome of <i>L. multiflorum</i> under drought. We identified 41,141 DEGs in leaves, 35,559 DEGs in roots, respectively. Moreover, we identified 1243 alternative splicing events under drought. LmPIP5K9 produced two different transcripts with opposite expression patterns, possibly through the phospholipid signaling pathway or the negatively regulated sugar-mediated root growth response to drought stress, respectively. Additionally, 13,079 transcription factors in 90 families were obtained. An in-depth analysis of R2R3-MYB gene family members was performed to preliminarily demonstrate their functions by utilizing subcellular localization and overexpression in yeast. Our data make a significant contribution to the genetics of <i>L. multiflorum</i>, offering a current understanding of plant adaptation to drought stress.https://www.mdpi.com/1422-0067/23/14/7921<i>Lolium multiflorum</i>SMRT-Seqdrought stressPacBioR2R3-MYB |
| spellingShingle | Qiuxu Liu Fangyan Wang Yang Shuai Linkai Huang Xinquan Zhang Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> International Journal of Molecular Sciences <i>Lolium multiflorum</i> SMRT-Seq drought stress PacBio R2R3-MYB |
| title | Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> |
| title_full | Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> |
| title_fullStr | Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> |
| title_full_unstemmed | Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> |
| title_short | Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of <i>Lolium multiflorum</i> |
| title_sort | integrated analysis of single molecule real time sequencing and next generation sequencing eveals insights into drought tolerance mechanism of i lolium multiflorum i |
| topic | <i>Lolium multiflorum</i> SMRT-Seq drought stress PacBio R2R3-MYB |
| url | https://www.mdpi.com/1422-0067/23/14/7921 |
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