A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila
Background Previous studies have proven that bag-of-marbles (bam) plays a pivotal role in promoting early germ cell differentiation in Drosophila ovary. However, whether it functions in regulating the metabolic state of the host remains largely unknown. Methods We utilized GC-MS, qPCR, and some clas...
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PeerJ Inc.
2022-10-01
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| Online Access: | https://peerj.com/articles/14145.pdf |
| _version_ | 1797425615485272064 |
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| author | Jiale Wang Yangyang Zhu Chao Zhang Renjie Duan Fanrui Kong Xianrui Zheng Yongzhi Hua |
| author_facet | Jiale Wang Yangyang Zhu Chao Zhang Renjie Duan Fanrui Kong Xianrui Zheng Yongzhi Hua |
| author_sort | Jiale Wang |
| collection | DOAJ |
| description | Background Previous studies have proven that bag-of-marbles (bam) plays a pivotal role in promoting early germ cell differentiation in Drosophila ovary. However, whether it functions in regulating the metabolic state of the host remains largely unknown. Methods We utilized GC-MS, qPCR, and some classical kits to examine various metabolic profiles and gut microbial composition in bam loss-of-function mutants and age-paired controls. We performed genetic manipulations to explore the tissue/organ-specific role of bam in regulating energy metabolism in Drosophila. The DSS-induced mouse colitis was generated to identify the role of Gm114, the mammalian homolog of bam, in modulating intestinal homeostasis. Results We show that loss of bam leads to an increased storage of energy in Drosophila. Silence of bam in intestines results in commensal microbial dysbiosis and metabolic dysfunction of the host. Moreover, recovery of bam expression in guts almost rescues the obese phenotype in bam loss-of-function mutants. Further examinations of mammalian Gm114 imply a similar biological function in regulating the intestinal homeostasis and energy storage with its Drosophila homolog bam. Conclusion Our studies uncover a novel biological function of bam/Gm114 in regulating the host lipid homeostasis. |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:18:39Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-8b1dae8ca16c48b9a2a78200bc32a6ff2023-12-02T21:55:34ZengPeerJ Inc.PeerJ2167-83592022-10-0110e1414510.7717/peerj.14145A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in DrosophilaJiale Wang0Yangyang Zhu1Chao Zhang2Renjie Duan3Fanrui Kong4Xianrui Zheng5Yongzhi Hua6Anhui Agricultural University, Hefei, ChinaAnhui Agricultural University, Hefei, ChinaAnhui Agricultural University, Hefei, ChinaAnhui Agricultural University, Hefei, ChinaAnhui Agricultural University, Hefei, ChinaZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, ChinaAnhui Agricultural University, Hefei, ChinaBackground Previous studies have proven that bag-of-marbles (bam) plays a pivotal role in promoting early germ cell differentiation in Drosophila ovary. However, whether it functions in regulating the metabolic state of the host remains largely unknown. Methods We utilized GC-MS, qPCR, and some classical kits to examine various metabolic profiles and gut microbial composition in bam loss-of-function mutants and age-paired controls. We performed genetic manipulations to explore the tissue/organ-specific role of bam in regulating energy metabolism in Drosophila. The DSS-induced mouse colitis was generated to identify the role of Gm114, the mammalian homolog of bam, in modulating intestinal homeostasis. Results We show that loss of bam leads to an increased storage of energy in Drosophila. Silence of bam in intestines results in commensal microbial dysbiosis and metabolic dysfunction of the host. Moreover, recovery of bam expression in guts almost rescues the obese phenotype in bam loss-of-function mutants. Further examinations of mammalian Gm114 imply a similar biological function in regulating the intestinal homeostasis and energy storage with its Drosophila homolog bam. Conclusion Our studies uncover a novel biological function of bam/Gm114 in regulating the host lipid homeostasis.https://peerj.com/articles/14145.pdfLipid storage and metabolismBag-of-marbles (bam)Gm114Gut microbiotaIntestinal homeostasis |
| spellingShingle | Jiale Wang Yangyang Zhu Chao Zhang Renjie Duan Fanrui Kong Xianrui Zheng Yongzhi Hua A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila PeerJ Lipid storage and metabolism Bag-of-marbles (bam) Gm114 Gut microbiota Intestinal homeostasis |
| title | A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila |
| title_full | A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila |
| title_fullStr | A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila |
| title_full_unstemmed | A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila |
| title_short | A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila |
| title_sort | conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in drosophila |
| topic | Lipid storage and metabolism Bag-of-marbles (bam) Gm114 Gut microbiota Intestinal homeostasis |
| url | https://peerj.com/articles/14145.pdf |
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