Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin
Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from pl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2019-08-01
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| Series: | Pharmaceutical Sciences and Research |
| Subjects: | |
| Online Access: | http://psr.ui.ac.id/index.php/journal/article/view/4510 |
| Summary: | Background: Skin aging is a complex natural process caused by both intrinsic or genetically
programmed aging and extrinsic aging caused by environmental factors, such as free radical.
The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural
compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability
to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max.
Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin
through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory
activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed
by 2,2’- azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay.
Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in
aging process. Results: ABTS- reducing activity assay showed that both compounds had great
ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had
low value of IC50
ABTS-reducing activity around 14.39 µg/ml better than genistein with IC50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin
had higher antioxidant and anti- collagenase activity compared to Genistein. |
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| ISSN: | 2407-2354 2477-0612 |