Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin
Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from pl...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2019-08-01
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| Series: | Pharmaceutical Sciences and Research |
| Subjects: | |
| Online Access: | http://psr.ui.ac.id/index.php/journal/article/view/4510 |
| _version_ | 1818656813718962176 |
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| author | Geeta Wahyu Setia Widodo Wahyu Widowati Chrismis Novalinda Ginting I Nyoman Ehrich Lister Adek Armansyah Ermi Girsang |
| author_facet | Geeta Wahyu Setia Widodo Wahyu Widowati Chrismis Novalinda Ginting I Nyoman Ehrich Lister Adek Armansyah Ermi Girsang |
| author_sort | Geeta |
| collection | DOAJ |
| description | Background: Skin aging is a complex natural process caused by both intrinsic or genetically
programmed aging and extrinsic aging caused by environmental factors, such as free radical.
The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural
compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability
to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max.
Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin
through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory
activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed
by 2,2’- azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay.
Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in
aging process. Results: ABTS- reducing activity assay showed that both compounds had great
ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had
low value of IC50
ABTS-reducing activity around 14.39 µg/ml better than genistein with IC50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin
had higher antioxidant and anti- collagenase activity compared to Genistein. |
| first_indexed | 2024-12-17T03:31:33Z |
| format | Article |
| id | doaj.art-8b21c34690ce4f93a38fdac9677486c5 |
| institution | Directory Open Access Journal |
| issn | 2407-2354 2477-0612 |
| language | English |
| last_indexed | 2024-12-17T03:31:33Z |
| publishDate | 2019-08-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Pharmaceutical Sciences and Research |
| spelling | doaj.art-8b21c34690ce4f93a38fdac9677486c52022-12-21T22:05:16ZengUniversitas IndonesiaPharmaceutical Sciences and Research2407-23542477-06122019-08-0162111117https://doi.org/10.7454/psr.v6i2.4510Comparison of Antioxidant and Anti-collagenase Activity of Genistein and EpicatechinGeeta0Wahyu Setia Widodo1Wahyu Widowati2Chrismis Novalinda Ginting 3I Nyoman Ehrich Lister4Adek Armansyah5Ermi Girsang6Master in Biomedical Science Program, Faculty of Medicine, Universitas Prima Indonesia, Medan, IndonesiaBiomolecular and Biomedicine Research Center, Aretha Media Utama, Bandung, IndonesiaFaculty of Medicine, Maranatha Christian University, Bandung, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan, IndonesiaMaster in Biomedical Science Program, Faculty of Medicine, Universitas Prima Indonesia, Medan, IndonesiaBackground: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max. Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed by 2,2’- azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay. Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in aging process. Results: ABTS- reducing activity assay showed that both compounds had great ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had low value of IC50 ABTS-reducing activity around 14.39 µg/ml better than genistein with IC50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin had higher antioxidant and anti- collagenase activity compared to Genistein.http://psr.ui.ac.id/index.php/journal/article/view/4510abts-reducing activityanti-collagenasegenisteinepicatechin |
| spellingShingle | Geeta Wahyu Setia Widodo Wahyu Widowati Chrismis Novalinda Ginting I Nyoman Ehrich Lister Adek Armansyah Ermi Girsang Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin Pharmaceutical Sciences and Research abts-reducing activity anti-collagenase genistein epicatechin |
| title | Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin |
| title_full | Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin |
| title_fullStr | Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin |
| title_full_unstemmed | Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin |
| title_short | Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin |
| title_sort | comparison of antioxidant and anti collagenase activity of genistein and epicatechin |
| topic | abts-reducing activity anti-collagenase genistein epicatechin |
| url | http://psr.ui.ac.id/index.php/journal/article/view/4510 |
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