Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

Summary: Here, we present a protocol for estimating nuclear transport parameters in single cells. We describe steps for performing four consecutive fluorescence recovery after photobleaching experiments, fitting the obtained data to an ordinary differential equations model, and statistical analysis...

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Bibliographic Details
Main Authors: Lucía Durrieu, Alan Bush, Alejandro Colman-Lerner
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Biophysics
Microscopy
Model Organisms
Systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000418

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