Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

Summary: Here, we present a protocol for estimating nuclear transport parameters in single cells. We describe steps for performing four consecutive fluorescence recovery after photobleaching experiments, fitting the obtained data to an ordinary differential equations model, and statistical analysis...

Полное описание

Библиографические подробности
Главные авторы:	Lucía Durrieu, Alan Bush, Alejandro Colman-Lerner
Формат:	Статья
Язык:	English
Опубликовано:	Elsevier 2024-03-01
Серии:	STAR Protocols
Предметы:	Biophysics Microscopy Model Organisms Systems biology
Online-ссылка:	http://www.sciencedirect.com/science/article/pii/S2666166724000418

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