Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens
Spaghetti meat (SM) and woody breast (WB) are breast muscle myopathies of broiler chickens, characterized by separation of myofibers and by fibrosis, respectively. This study sought to investigate the transcriptomic profiles of breast muscles affected by SM and WB. Targeted sampling was conducted on...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/14/2/176 |
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| author | Sunoh Che Phuc H. Pham Shai Barbut Dorothee Bienzle Leonardo Susta |
| author_facet | Sunoh Che Phuc H. Pham Shai Barbut Dorothee Bienzle Leonardo Susta |
| author_sort | Sunoh Che |
| collection | DOAJ |
| description | Spaghetti meat (SM) and woody breast (WB) are breast muscle myopathies of broiler chickens, characterized by separation of myofibers and by fibrosis, respectively. This study sought to investigate the transcriptomic profiles of breast muscles affected by SM and WB. Targeted sampling was conducted on a flock to obtain 10 WB, 10 SM, and 10 Normal <i>Pectoralis major</i> muscle samples from 37-day-old male chickens. Total RNA was extracted, cDNA was used for pair-end sequencing, and differentially expressed genes (DEGs) were determined by a false discovery rate of <0.1 and a >1.5-fold change. Principal component and heatmap cluster analyses showed that the SM and WB samples clustered together. No DEGs were observed between SM and WB fillets, while a total of 4018 and 2323 DEGs were found when comparing SM and WB, respectively, against Normal samples. In both the SM and WB samples, Gene Ontology terms associated with extracellular environment and immune response were enriched. The KEGG analysis showed enrichment of cytokine–cytokine receptor interaction and extracellular matrix–receptor interaction pathways in both myopathies. Although SM and WB are macroscopically different, the similar transcriptomic profiles suggest that these conditions may share a common pathogenesis. This is the first study to compare the transcriptomes of SM and WB, and it showed that, while both myopathies had profiles different from the normal breast muscle, SM and WB were similar, with comparable enriched metabolic pathways and processes despite presenting markedly different macroscopic features. |
| first_indexed | 2024-03-08T11:08:34Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:08:34Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-8b227a3accc642cda237f9e3a9d413712024-01-26T14:30:03ZengMDPI AGAnimals2076-26152024-01-0114217610.3390/ani14020176Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler ChickensSunoh Che0Phuc H. Pham1Shai Barbut2Dorothee Bienzle3Leonardo Susta4Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G2W1, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G2W1, CanadaDepartment of Food Science, Ontario Agricultural College, University of Guelph, Guelph, ON N1G2W1, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G2W1, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G2W1, CanadaSpaghetti meat (SM) and woody breast (WB) are breast muscle myopathies of broiler chickens, characterized by separation of myofibers and by fibrosis, respectively. This study sought to investigate the transcriptomic profiles of breast muscles affected by SM and WB. Targeted sampling was conducted on a flock to obtain 10 WB, 10 SM, and 10 Normal <i>Pectoralis major</i> muscle samples from 37-day-old male chickens. Total RNA was extracted, cDNA was used for pair-end sequencing, and differentially expressed genes (DEGs) were determined by a false discovery rate of <0.1 and a >1.5-fold change. Principal component and heatmap cluster analyses showed that the SM and WB samples clustered together. No DEGs were observed between SM and WB fillets, while a total of 4018 and 2323 DEGs were found when comparing SM and WB, respectively, against Normal samples. In both the SM and WB samples, Gene Ontology terms associated with extracellular environment and immune response were enriched. The KEGG analysis showed enrichment of cytokine–cytokine receptor interaction and extracellular matrix–receptor interaction pathways in both myopathies. Although SM and WB are macroscopically different, the similar transcriptomic profiles suggest that these conditions may share a common pathogenesis. This is the first study to compare the transcriptomes of SM and WB, and it showed that, while both myopathies had profiles different from the normal breast muscle, SM and WB were similar, with comparable enriched metabolic pathways and processes despite presenting markedly different macroscopic features.https://www.mdpi.com/2076-2615/14/2/176broiler chicken myopathydifferentially expressed genespathogenesispathway analysisRNA sequencing |
| spellingShingle | Sunoh Che Phuc H. Pham Shai Barbut Dorothee Bienzle Leonardo Susta Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens Animals broiler chicken myopathy differentially expressed genes pathogenesis pathway analysis RNA sequencing |
| title | Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens |
| title_full | Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens |
| title_fullStr | Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens |
| title_full_unstemmed | Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens |
| title_short | Transcriptomic Profiles of <i>Pectoralis major</i> Muscles Affected by Spaghetti Meat and Woody Breast in Broiler Chickens |
| title_sort | transcriptomic profiles of i pectoralis major i muscles affected by spaghetti meat and woody breast in broiler chickens |
| topic | broiler chicken myopathy differentially expressed genes pathogenesis pathway analysis RNA sequencing |
| url | https://www.mdpi.com/2076-2615/14/2/176 |
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