Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture
Contamination by the predatory zooplankton <i>Poterioochromonas malhamensis</i> is one of the major threats that causes catastrophic damage to commercial-scale microalgal cultivation. However, knowledge of how to manage predator contamination is limited. Previously, we established a phos...
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MDPI AG
2021-07-01
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| Series: | Plants |
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| author | Narumi Toda Hiroki Murakami Akihiro Kanbara Akio Kuroda Ryuichi Hirota |
| author_facet | Narumi Toda Hiroki Murakami Akihiro Kanbara Akio Kuroda Ryuichi Hirota |
| author_sort | Narumi Toda |
| collection | DOAJ |
| description | Contamination by the predatory zooplankton <i>Poterioochromonas malhamensis</i> is one of the major threats that causes catastrophic damage to commercial-scale microalgal cultivation. However, knowledge of how to manage predator contamination is limited. Previously, we established a phosphite (Pt)-based culture system by engineering <i>Synechococcus elongatus</i>, which exerted a competitive growth advantage against microbial contaminants that compete with phosphate source. Here, we examined whether Pt is effective in suppressing predator-type contamination. Co-culture experiment of <i>Synechococcus</i> with isolated <i>P. malhamensis</i> revealed that, although an addition of Pt at low concentrations up to 2.0 mM was not effective, increased dosage of Pt (~20 mM) resulted in the reduced grazing impact of <i>P. malhamensis</i>. By using unsterilized raw environmental water collected from rivers or ponds, we found that the suppression effect of Pt was dependent on the type of environmental water used. Eukaryotic microbial community analysis of the cultures using environmental water samples revealed that <i>Paraphysomonas</i>, a colorless Chrysophyceae, emerged and dominated under high-Pt conditions, suggesting that <i>Paraphysomonas</i> is insensitive to Pt compared to <i>P. malhamensis</i>. These findings may provide a clue for developing a strategy to reduce the impact of grazer contamination in commercial-scale microalgal cultivation. |
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| language | English |
| last_indexed | 2024-03-10T09:28:25Z |
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| spelling | doaj.art-8b22c96e34a64b248ad1efaae40881b92023-11-22T04:42:56ZengMDPI AGPlants2223-77472021-07-01107136110.3390/plants10071361Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial CultureNarumi Toda0Hiroki Murakami1Akihiro Kanbara2Akio Kuroda3Ryuichi Hirota4Unit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8528, JapanUnit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8528, JapanUnit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8528, JapanUnit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8528, JapanUnit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8528, JapanContamination by the predatory zooplankton <i>Poterioochromonas malhamensis</i> is one of the major threats that causes catastrophic damage to commercial-scale microalgal cultivation. However, knowledge of how to manage predator contamination is limited. Previously, we established a phosphite (Pt)-based culture system by engineering <i>Synechococcus elongatus</i>, which exerted a competitive growth advantage against microbial contaminants that compete with phosphate source. Here, we examined whether Pt is effective in suppressing predator-type contamination. Co-culture experiment of <i>Synechococcus</i> with isolated <i>P. malhamensis</i> revealed that, although an addition of Pt at low concentrations up to 2.0 mM was not effective, increased dosage of Pt (~20 mM) resulted in the reduced grazing impact of <i>P. malhamensis</i>. By using unsterilized raw environmental water collected from rivers or ponds, we found that the suppression effect of Pt was dependent on the type of environmental water used. Eukaryotic microbial community analysis of the cultures using environmental water samples revealed that <i>Paraphysomonas</i>, a colorless Chrysophyceae, emerged and dominated under high-Pt conditions, suggesting that <i>Paraphysomonas</i> is insensitive to Pt compared to <i>P. malhamensis</i>. These findings may provide a clue for developing a strategy to reduce the impact of grazer contamination in commercial-scale microalgal cultivation.https://www.mdpi.com/2223-7747/10/7/1361microalgal cultivationcyanobacteriacontaminationpredatorChrysophyceae<i>Poterioochromonas</i> |
| spellingShingle | Narumi Toda Hiroki Murakami Akihiro Kanbara Akio Kuroda Ryuichi Hirota Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture Plants microalgal cultivation cyanobacteria contamination predator Chrysophyceae <i>Poterioochromonas</i> |
| title | Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture |
| title_full | Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture |
| title_fullStr | Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture |
| title_full_unstemmed | Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture |
| title_short | Phosphite Reduces the Predation Impact of <em>Poterioochromonas</em> <em>malhamensis</em> on Cyanobacterial Culture |
| title_sort | phosphite reduces the predation impact of em poterioochromonas em em malhamensis em on cyanobacterial culture |
| topic | microalgal cultivation cyanobacteria contamination predator Chrysophyceae <i>Poterioochromonas</i> |
| url | https://www.mdpi.com/2223-7747/10/7/1361 |
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